Recombinant Human MMP-8 (C-10His, HEK293)是人源蛋白，在HEK293人源细胞中表达，带有C端10xHis标签。该蛋白为基质金属蛋白酶8（MMP8，又称中性粒细胞胶原酶），主要在嗜中性粒细胞中表达并储存于特定颗粒中，可被多种因子刺激释放，具有降解I、II、III型胶原、明胶、纤连蛋白等多种胞外基质成分的能力，参与类风湿性关节炎等疾病的病理过程，且高度糖基化。
Human MMP-8 (C-10His, HEK293)/人MMP-8蛋白(C-10His, HEK293) 化学结构 CAS号：N/A
Human MMP-8 (C-10His, HEK293)/人MMP-8蛋白(C-10His, HEK293) 3D分子结构 CAS号：N/A
Human MMP-8 (C-10His, HEK293)/人MMP-8蛋白(C-10His, HEK293) 质控信息
Human MMP-8 (C-10His, HEK293)/人MMP-8蛋白(C-10His, HEK293) 生物活性
描述
Matrix metalloproteinases (MMPs) are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP8(neutrophil collagenase) is expressed in neutrophils, where it is stored in specific granules. MMP8 release from the neutrophils is stimulated by various factors such as interleukins 1 and 8, TNF-α and GM-CSF. MMP8 is capable of cleaving types I, II and III triple-helical collagen, gelatin peptides, fibronectin, proteoglycans, aggrecan, serpins, β-casein and peptides such as angiotensin and substance P. In addition to its function in phagocytosis, MMP8 has a high capacity for infiltrating connective tissue, and is implicated in the breakdown of the extracellular matrix in diseases such as rheumatoid arthritis. MMP8 is heavily glycosylated.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human MMP-8 (C-10His, HEK293)/人MMP-8蛋白(C-10His, HEK293) 技术信息
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