货号:A2900823
同义名:
重组人MIF(N-6His)蛋白(E.coli, tag free)
/ Recombinant Human MIF(N-6His); Macrophage migration inhibitory factor
Recombinant Human MIF(N-6His)是人源蛋白,在大肠杆菌中表达,带有N端6×His标签。该蛋白为12.5 kDa、115个氨基酸组成的非糖基化多肽,无信号肽,通过ABCA1转运体以非经典途径分泌。作为促炎细胞因子,参与先天免疫应答,调控巨噬细胞功能,拮抗糖皮质激素的抗炎作用,具有苯丙酮酸和多巴色素互变异构酶活性,但生理底物尚不明确。


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| 描述 | Human MIF is a 12.5 kDa, 115 amino acid (aa) nonglycosylated polypeptide that is synthesized without asignal sequence .Secretion occurs nonclassically via an ABCA1 transporter.Pro-inflammatory cytokine.Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites ofinflammation suggests a role as mediator in regulating the function of acrophages in host defense.Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase anddopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clearwhether the tautomerase activity has any physiological relevance, and whether it is important for cytokineactivity. |
| 蛋白种属 | Human |
| 表达系统 | E.coli |
| 标签 | N-6His |
| 蛋白表达区间 | Met1-Ala115 |
| 蛋白编号 | P14174 |
| 内毒素 | < 1 EU/µg as determined by LAL test. |
| 剂型 | 液体 |
| 剂型描述 | Supplied as a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 50% Glycerol, pH7.4. |
| 复溶 | 如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
| CAS号 | N/A |
| 分子量 | 14.6 KDa |
| SMILES Code | NONE |
| 别名 | 重组人MIF(N-6His)蛋白(E.coli, tag free) ;Recombinant Human MIF(N-6His); Macrophage migration inhibitory factor; MIF; MMIF; Glycosylation-inhibiting factor; GLIF; L-dopachrome tautomerase; Phenylpyruvate tautomerase |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C:3-6个月-80°C:12个月 |
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