Human Lefty-A (N-6His)/人Lefty-A蛋白(HEK293, N-6His) 质控信息
Human Lefty-A (N-6His)/人Lefty-A蛋白(HEK293, N-6His) 生物活性
描述
产品名称：Recombinant Human Lefty-A (N-6His)理论分子量：39.1 KDa蛋白种属：Human表达系统：Human Cells蛋白表达区间：Phe78-Pro366标签：N-6His剂型：干粉纯度：≥90% as determined by reducing SDS-PAGE.储存条件：冻干蛋白应储存在≤-20°C，收到后可稳定保存一年。
溶解后的蛋白溶液可以在2-8°C下保存2-7天。如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。产品背景：Left-right determination factor 2(LEFTY2) is a secreted protein which belongs to the TGF-beta family. Lefty was first identified in a screen for undifferentiated cell-specific cDNAs from the P19 mouse embryonal carcinoma cells. Its mRNA expression on the left side of the developing embryo earned the name “Lefty”. The human orthologue was initially identified as Ebaf, Endometrial bleeding associated factor. Lefty contains the six cysteine residues that are conserved among TGF-β related proteins and that are necessary to form the cysteineknot structure. Its function in patterning left-right asymmetry of the developing organ systems such as the heart and lung is consistent in all vertebrate species examined. Lefty acts as an antagonist to Nodal signaling, potentially by competing for binding to a common receptor. It may play a role in endometrial bleeding.
Human Lefty-A (N-6His)/人Lefty-A蛋白(HEK293, N-6His) 技术信息
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