Human KRAS(G12C, N-6His, E.coli)/人KRAS(G12C)蛋白(N-6His, E.coli)
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货号：A2925522同义名：
重组人KRAS(G12C)蛋白(N-6His, E.coli)
/ Recombinant Human KRAS(G12C, N-6His, E.coli); Ki-Ras
Recombinant Human KRAS(G12C, N-6His, E.coli)是人类K-Ras蛋白，在大肠杆菌中表达，带有N端6×His标签。该蛋白属于小GTP酶超家族Ras家族，是一种GTP酶，作为信号转导通路中的分子开关，调控细胞增殖，并在结直肠癌等肿瘤发生发展中起促癌作用，其突变与多种癌症密切相关。
Human KRAS(G12C, N-6His, E.coli)/人KRAS(G12C)蛋白(N-6His, E.coli) 化学结构 CAS号：N/A
Human KRAS(G12C, N-6His, E.coli)/人KRAS(G12C)蛋白(N-6His, E.coli) 3D分子结构 CAS号：N/A
Human KRAS(G12C, N-6His, E.coli)/人KRAS(G12C)蛋白(N-6His, E.coli) 质控信息
Human KRAS(G12C, N-6His, E.coli)/人KRAS(G12C)蛋白(N-6His, E.coli) 生物活性
描述
K-Ras belongs to the small GTPase superfamily, Ras family. As other members of the Ras family, K-Ras is a GTPase and is an early player in many signal transduction pathways. It is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. K-Ras functions as a molecular on/off switch. Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation. Plays a role in promoting oncogenic events by inducing transcriptional silencing of tumor suppressor genes (TSGs) in colorectal cancer (CRC) cells in a ZNF304-dependent manner. Besides essential function in normal tissue signaling, the mutation of a K-Ras gene is an essential step in the development of many cancers. Several germline K-Ras mutations have been found to be associated with Noonan syndrome[4] and cardio-facio-cutaneous syndrome. Somatic K-Ras mutations are found at high rates in Leukemias, colon cancer, pancreatic cancer and lung cancer.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human KRAS(G12C, N-6His, E.coli)/人KRAS(G12C)蛋白(N-6His, E.coli) 技术信息
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