Human IL-33/人IL-33蛋白(E.coli, tag free)
{[allProObj[0].p_purity_real_show]}
货号：A2900796同义名：
重组人IL-33蛋白(E.coli, tag free)
/ Recombinant Human IL-33; Interleukin-33
Recombinant Human IL-33是人源白细胞介素-33蛋白，在大肠杆菌中表达，为无标签形式。该蛋白是IL-1家族的促炎核因子，具有细胞内和细胞外活性，全长270个氨基酸，主要表达于人体高内皮静脉内皮细胞，其C端成熟片段可结合并激活ST2受体，触发NF-κB和MAPK信号通路，诱导Th2型细胞因子产生，与黏膜免疫病理密切相关。
Human IL-33/人IL-33蛋白(E.coli, tag free) 化学结构 CAS号：N/A
Human IL-33/人IL-33蛋白(E.coli, tag free) 3D分子结构 CAS号：N/A
Interleukin-33 (IL-33) was initially discovered as a nuclear factor NF-HEV abundantly expressed in high endothelial venules. It is a 30-32 kD pro-inflammatory protein with intracellular and extracellular activities and a chromatin-associated cytokine of the IL-1 family with high sequence and structural similarity to IL-1 and IL-18. IL-33 is highly and selectively expressed by high endothelial venule endothelial cells (HEVECs) in human tonsils, Peyers's patches, and lymph nodes. It contains a bipartite nuclear localization signal at the C-terminus, and is targeted to the nucleus when ectopically expressed in human umbilical vein endothelial cells (HUVECs) and HeLa cells. The C-terminal fragment, corresponding to mature IL-33, binds and triggers signaling. IL-33 mediates its biological effects via Toll-interleukin 1 (IL-1) receptor (TIR) domain-containing receptor ST2, activates NF-kappaB and MAP kinases, and drives production of T(H)2-associated cytokines from in vitro polarized T(H)2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. Human IL-33 is 270 amino acids in length.
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human IL-33/人IL-33蛋白(E.coli, tag free) 细胞实验
Cell Line
Concentration
Treated Time
Description
References
T84 cells
10 ng/mL
48 hours
To evaluate the effect of IL-33 on epithelial barrier function. Results showed that IL-33 augmented TER in T84 cells via ERK1/2 signaling pathway.
Inflamm Bowel Dis. 2015 Dec;21(12):2737-46
Human IL-33/人IL-33蛋白(E.coli, tag free) 技术信息
CAS号
N/A
分子量
18.1 KDa
SMILES Code
NONE
别名
重组人IL-33蛋白(E.coli, tag free)
;Recombinant Human IL-33; Interleukin-33; IL-33; Interleukin-1 Family Member 11; IL-1F11; Nuclear Factor From High Endothelial Venules; NF-HEV; IL33; C9orf26; IL1F11; NFHEV
运输
蓝冰
存储条件
In solvent-20°C:3-6个月-80°C:12个月
Pure formSealed in dry, store in freezer, under -20°C
最近浏览的产品
Tags:
Human IL-33/人IL-33蛋白(E.coli, tag free)
|Human IL-33/人IL-33蛋白(E.coli, tag free)
| 重组人IL-33蛋白(E.coli, tag free)
| Recombinant Human IL-33
| Interleukin-33
| IL-33
| Interleukin-1 Family Member 11
| IL-1F11
| Nuclear Factor From High Endothelial Venules
| NF-HEV
| IL33
| C9orf26
| IL1F11
| NFHEV
| N/A
| Interleukin & Receptors
| Human IL-33/人IL-33蛋白(E.coli, tag free) 纯度/质量文件
| Human IL-33/人IL-33蛋白(E.coli, tag free) 生物活性
| Human IL-33/人IL-33蛋白(E.coli, tag free) 技术信息
搜索
