货号:A2924166
同义名:
重组人IGFBP-4蛋白(C-6His, HEK293)
/ Recombinant Human IGFBP-4 (C-6His, HEK293); Insulin-Like Growth Factor-Binding Protein 4
Recombinant Human IGFBP-4 (C-6His, HEK293) 是人源胰岛素样生长因子结合蛋白4,由HEK293细胞表达,带有C端6×His标签。该蛋白分子量约24 kDa,可高亲和力结合IGF-1和IGF-2,尤其对IGF-2亲和更强,具有IGFBP N端结构域和甲状腺球蛋白类型1结构域,能抑制IGF的生物活性,调节其半衰期及对细胞生长的促进或抑制作用。


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| 描述 | Insulin-like growth factor binding protein 4 (IGFBP-4) is a 24 kDa protein that binds insulin-like growth factor 1 (IGF-1) and IGF-2 with high affinity and inhibits IGF action in vitro. All members of the IGFBP family can bind IGF-I and IGF-II with about equal affinity, but IGFBP-4 binds IGF2 more than IGF1. It contains IGFBP N-terminal domain and thyroglobulin type-1 domain. IGFBP-4 is induced by forskolin and N6, O2’dibutyryl sdenosine 3’, or 5’-cyclic monophosphate. The IGF-binding proteins can prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. |
| 蛋白种属 | Human |
| 表达系统 | Human Cells |
| 标签 | C-6His |
| 蛋白表达区间 | Asp22-Glu258 |
| 蛋白编号 | P22692 |
| 内毒素 | < 1 EU/µg as determined by LAL test. |
| 剂型 | 干粉 |
| 剂型描述 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。 分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
| CAS号 | N/A |
| 分子量 | 27.01 KDa |
| SMILES Code | NONE |
| 别名 | 重组人IGFBP-4蛋白(C-6His, HEK293) ;Recombinant Human IGFBP-4 (C-6His, HEK293); Insulin-Like Growth Factor-Binding Protein 4; IBP-4; IGF-Binding Protein 4; IGFBP-4; IGFBP4; IBP4 |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C: 3-6个月 -80°C: 12个月 Pure form Sealed in dry, store in freezer, under -20°C |
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