Recombinant Human HER2 (C-6His, HEK293)是人源HER2蛋白，在HEK293人源细胞中表达，带有C端6×His标签。该蛋白是一种膜糖蛋白，属于表皮生长因子受体家族，参与发育、细胞增殖与分化，与多种癌症的恶性程度及不良预后相关，通过二聚化激活，并与EGFR、ERBB3和ERBB4形成异源二聚体，影响转录调控及蛋白合成，促进细胞生长。
Human HER2 (C-6His, HEK293)/人HER2蛋白(C-6His, HEK293) 化学结构 CAS号：N/A
Human HER2 (C-6His, HEK293)/人HER2蛋白(C-6His, HEK293) 3D分子结构 CAS号：N/A
Human HER2 (C-6His, HEK293)/人HER2蛋白(C-6His, HEK293) 质控信息
Human HER2 (C-6His, HEK293)/人HER2蛋白(C-6His, HEK293) 生物活性
描述
Human epidermal growth factor receptor 2 (HER2) is a type of membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 plays a key role in development, cell proliferation and differentiation. HER2 has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on. HER2 is activated by dimerization and not activated by EGF, TGF-alpha and amphiregulin. Interaction with PTK6 increases its intrinsic kinase activity.It is heterodimer with EGFR, ERBB3 and ERBB4. HER2 associates with the 5'-TCAAATTC-3' sequence in the PTGS2/COX-2 promoter and activates its transcription. It implicated in transcriptional activation of CDKN1A and the function of the protein involves STAT3 and SRC. And also it involved in the transcription of rRNA genes by RNA Pol I and enhances protein synthesis and cell growth.
Lyophilized from a 0.2 μm filtered solution of PBS,pH7.4
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human HER2 (C-6His, HEK293)/人HER2蛋白(C-6His, HEK293) 技术信息
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