货号:A2923971
同义名:
重组人FGFb蛋白(155AA, Tag free, E.coli)
/ Recombinant Human FGFb (155AA, E.coli); Fibroblast growth factor 2
Recombinant Human FGFb(155AA,E.coli)是人源蛋白,在大肠杆菌中表达,无标签。该蛋白即成纤维细胞生长因子2(FGF2),属于硫酸乙酰肝素结合生长因子家族,由上皮、肿瘤等细胞产生,参与发育过程,调控分化、增殖和迁移,是体外培养胚胎干细胞不诱导分化的关键因子,对硫酸乙酰肝素有高亲和力,其结合是激活FGFR酪氨酸激酶的关键步骤。


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| 描述 | Fibroblast growth factor 2(FGF2) is a secreted protein and belongs to the heparin-binding growth factors family. FGF2 is produced by epithelial, tumor and other cell types. It involved in developmental processes and regulates differentiation, proliferation, and migration, FGF2 is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF2 has a high affinity for heparan sulfate and binding is a step in the FGF basic activation of FGFR tyrosine kinase. |
| 蛋白种属 | Human |
| 表达系统 | E.coli |
| 标签 | Tag free |
| 蛋白表达区间 | Met134-Ser288 |
| 蛋白编号 | P09038-4 |
| 内毒素 | < 1 EU/µg as determined by LAL test. |
| 剂型 | 干粉 |
| 剂型描述 | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 8% Trehalose, 2% Mannitol, 0.05% Tween80, 2mM EDTA, pH8.0. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。 分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
| CAS号 | N/A |
| 分子量 | 17.3 KDa |
| SMILES Code | NONE |
| 别名 | 重组人FGFb蛋白(155AA, Tag free, E.coli) ;Recombinant Human FGFb (155AA, E.coli); Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; Bfgf; Heparin-binding growth factor 2; HBGF-2; FGF2; FGFB |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C: 3-6个月 -80°C: 12个月 Pure form Sealed in dry, store in freezer, under -20°C |
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