Recombinant Human FGF-21 (N-6His) 是人源成纤维细胞生长因子21重组蛋白，在大肠杆菌中表达，带有N端6×His标签。该蛋白属于FGF家族，参与调控组织发育、再生及细胞增殖分化，可促进脂肪细胞葡萄糖摄取，降低血糖与甘油三酯水平，是治疗肥胖与糖尿病的潜在靶点，由肝细胞响应游离脂肪酸刺激而分泌。
Human FGF-21 (N-6His)/人FGF-21蛋白(E.coli, N-6His) 化学结构 CAS号：N/A
Human FGF-21 (N-6His)/人FGF-21蛋白(E.coli, N-6His) 3D分子结构 CAS号：N/A
Human FGF-21 (N-6His)/人FGF-21蛋白(E.coli, N-6His) 质控信息
Human FGF-21 (N-6His)/人FGF-21蛋白(E.coli, N-6His) 生物活性
描述
Fibroblast Growth Factor 21 (FGF21) is a growth factor that belongs to the FGF family. FGF family proteins play a central role during prenatal development and postnatal growth and regeneration of mamy tissues, by promoting cellular proliferation and differentiation. FGF21 is a potent activator of glucose uptake on adipocytes, protects animal from diet-induced obesity when overexpression in transgenic mice, and lower blood glucose and triglyceride levels when therapeutically adiministered to diabetic redents. FGF21 is produced by hepatocytes in reponse to free fatty acid stimulation of a PPARa/RXR dimeric complex. This situation occurs clinically during starvation, or following the ingestionof a highly-fat/low-carbohydrate diet.Upon FGF21 secretion, white adipose tissue is induced to release free fatty acids from triglyceride stores. Once free fatty acid reach hepatocytes, they are oxidized and reduced to acetyl-CoA. The acetyl-CoA is recombined into 4-carbon ketone bodies, release, and transported to peripheral tissue for TCA processing and energy generation.
Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 2mM EDTA, pH 9.0 .
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human FGF-21 (N-6His)/人FGF-21蛋白(E.coli, N-6His) 技术信息
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