Recombinant Human EphA1 (C-6His, HEK293)是人源EphA1蛋白，在HEK293细胞中表达，带有C端6×His标签。该蛋白为糖基化的跨膜受体酪氨酸激酶，属于Eph家族成员，参与调控细胞迁移、组织形态发生及肿瘤进展，其正向与反向信号通路在Eph-Ephrin相互作用中发挥关键作用，广泛表达于分化上皮细胞，与多种人类癌症的侵袭性相关。
Human EphA1 (C-6His, HEK293)/人EphA1蛋白(C-6His, HEK293) 化学结构 CAS号：N/A
Human EphA1 (C-6His, HEK293)/人EphA1蛋白(C-6His, HEK293) 3D分子结构 CAS号：N/A
Human EphA1 (C-6His, HEK293)/人EphA1蛋白(C-6His, HEK293) 质控信息
Human EphA1 (C-6His, HEK293)/人EphA1蛋白(C-6His, HEK293) 生物活性
描述
Ephrin type-A receptor 1/EphA1 is a glycosylated member of the Eph family of transmembrane receptor tyrosine kinases. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression. EphA1 can form pH sensitive cishomodimers on the cell surface. Membrane-bound or clustered Ephrin ligands interact with EphA1 and activate its kinase domain which is capable of Ser, Thr, and Tyr phosphorylation. Reverse signaling is propagated through the Ephrin ligand. EphA1 is widely expressed in differentiated epithelial cells, particularly in bone marrow, spleen, thymus, and testes. EphA1 is upregulated or downregulated in a variety of human carcinomas and is implicated in tumor invasiveness.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human EphA1 (C-6His, HEK293)/人EphA1蛋白(C-6His, HEK293) 技术信息
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