Recombinant Human EGFR (C-Fc, HEK293)是人源蛋白，在HEK293人源细胞中表达，带有C-Fc标签。该蛋白是表皮生长因子家族的跨膜糖蛋白受体，属于蛋白激酶超家族，可结合表皮生长因子，诱导受体二聚化和酪氨酸自磷酸化，促进细胞增殖，并激活RAS-RAF-MEK-ERK、PI3K-AKT、PLCγ-PKC、STATs及NF-κB等下游信号通路，还能磷酸化RGS16等蛋白，参与多种细胞信号调控。
Human EGFR (C-Fc, HEK293)/人EGFR蛋白(C-Fc, HEK293) 化学结构 CAS号：N/A
Human EGFR (C-Fc, HEK293)/人EGFR蛋白(C-Fc, HEK293) 3D分子结构 CAS号：N/A
Human EGFR (C-Fc, HEK293)/人EGFR蛋白(C-Fc, HEK293) 质控信息
Human EGFR (C-Fc, HEK293)/人EGFR蛋白(C-Fc, HEK293) 生物活性
描述
EGFR is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. May also activate the NF-kappa-B signaling cascade. Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human EGFR (C-Fc, HEK293)/人EGFR蛋白(C-Fc, HEK293) 技术信息
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