货号:A2925775
同义名:
重组人DNA PolymeraseBeta蛋白(C-6His, E.coli)
/ Recombinant Human DNA PolymeraseBeta (C-6His, E.coli); DNA Polymerase Beta
Recombinant Human DNA Polymerase Beta (C-6His, E.coli) 是人源DNA聚合酶β,在大肠杆菌中表达,带有C端6×His标签。该蛋白在细胞中组成性表达,参与碱基切除修复,填补DNA缺口,具有5'-脱氧核糖-5-磷酸裂解酶活性和DNA聚合酶活性,以非连续方式合成DNA,其活性不受阿菲迪霉素抑制。


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| 描述 | Human DNA polymerase β is constitutively expressed in cells. It fills in gaps in DNA that are formed following base excision repair. Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. It conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. The activity cannot be affected by Aphidicolin, which is an inhibitor of DNA polymerase β. |
| 蛋白种属 | Human |
| 表达系统 | E.coli |
| 标签 | C-6His |
| 蛋白表达区间 | Ser2-Glu335 |
| 蛋白编号 | P06746 |
| 内毒素 | < 1 EU/µg as determined by LAL test. |
| 剂型 | 液体 |
| 剂型描述 | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM DTT, 1mM EDTA, 50% Glycerol, pH 7.8. |
| 复溶 | 如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
| CAS号 | N/A |
| 分子量 | 39.23 KDa |
| SMILES Code | NONE |
| 别名 | 重组人DNA PolymeraseBeta蛋白(C-6His, E.coli) ;Recombinant Human DNA PolymeraseBeta (C-6His, E.coli); DNA Polymerase Beta; POLB |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C:3-6个月-80°C:12个月 |
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