Recombinant Human CXCL4 (C-6His)是人类来源的重组蛋白，在人源细胞中表达，带有C端6×His标签。该蛋白为趋化因子CXCL4的重组形式，主要由巨核细胞表达并储存于血小板α颗粒中，具有多个肝素结合位点，以四聚体形式发挥活性，主要介导中性粒细胞、成纤维细胞和单核细胞的趋化作用，参与炎症与创伤修复，并通过结合肝素样分子调节凝血功能。
Human CXCL4 (C-6His)/人CXCL4蛋白(HEK293, C-6His) 化学结构 CAS号：N/A
Human CXCL4 (C-6His)/人CXCL4蛋白(HEK293, C-6His) 3D分子结构 CAS号：N/A
Human CXCL4 (C-6His)/人CXCL4蛋白(HEK293, C-6His) 质控信息
Human CXCL4 (C-6His)/人CXCL4蛋白(HEK293, C-6His) 生物活性
描述
Human Chemokine (C-X-C motif) Ligand 4 (CXCL4) is expressed in megakaryocytes and stored in the alpha-granules of platelets. CXCL4 contains several heparin-binding sites at the C-terminal region and binds heparin with high affinity. The active CXCL4 protein is a tetramer. Human and mouse CXCL4 share 64% sequence identity. CXCL4 is chemotactic for neutrophils, fibroblasts and monocytes and plays a critical role in inflammation and wound repair. CXCL4 functions via a splice variant of the chemokine receptor CXCR3, known as CXCR3B. The major physiologic role of CXCL4 appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes.
Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 5% Trehalose, 5% Mannitol, 1mM EDTA, 0.02% Tween 80, pH6.0.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human CXCL4 (C-6His)/人CXCL4蛋白(HEK293, C-6His) 技术信息
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