Human CPA2 (C-6His, HEK293)/人CPA2蛋白(C-6His, HEK293) 质控信息
Human CPA2 (C-6His, HEK293)/人CPA2蛋白(C-6His, HEK293) 生物活性
描述
Carboxypeptidase A2 (CPA) is a secreted pancreatic procarboxy-peptidase that cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E, and is involved in zymogen inhibition.
Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 10% Trehalose, 2% Mannitol, 0.05% Tween 80, pH7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human CPA2 (C-6His, HEK293)/人CPA2蛋白(C-6His, HEK293) 技术信息
CAS号
N/A
分子量
45.91 KDa
SMILES Code
NONE
别名
重组人CPA2蛋白(C-6His, HEK293)
;Recombinant Human CPA2 (C-6His, HEK293); Carboxypeptidase A2; CPA2
运输
蓝冰
存储条件
In solvent-20°C:3-6个月-80°C:12个月
Pure formSealed in dry, store in freezer, under -20°C
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