Recombinant Human CD99 (C-Fc, HEK293)是人类CD99蛋白，在人源细胞中表达，带有C端Fc标签。该蛋白是一种I型跨膜糖蛋白，属于CD99家族，其胞外域无明确结构模体，胞内区具有信号转导功能，参与调控中性粒细胞跨内皮迁移等炎症过程，并存在与不同生物学效应相关的I型和II型异构体。
Human CD99 (C-Fc, HEK293)/人CD99蛋白(C-Fc, HEK293) 化学结构 CAS号：N/A
Human CD99 (C-Fc, HEK293)/人CD99蛋白(C-Fc, HEK293) 3D分子结构 CAS号：N/A
Human CD99 (C-Fc, HEK293)/人CD99蛋白(C-Fc, HEK293) 质控信息
Human CD99 (C-Fc, HEK293)/人CD99蛋白(C-Fc, HEK293) 生物活性
描述
CD99 is a type I transmembrane glycoprotein and the founding member of the CD99 family of molecules. The extracellular domain of CD99 contains no identifiable motifs, its cytoplasmic region, although short, does have signal transduction capability. Cells known to express CD99 include fibroblasts, neutrophils, T cells, double positive thymocytes, CD34+ stem cells, monocytes and endothelial cells. Two types of CD99 isoforms have been classified. Native human CD99 is referred to as the long, or type I isoform. The best studied type II isoform shows an Asp-Gly substitution for the C terminal 27 amino acids. The type I and II isoforms have distinctive signal transduction pathways (FAKsrc for type I PI3K plus srcERK1/2 for type II), and mediate clearly different biological outcomes. Homophilic interaction between CD99 on the neutrophil and CD99 on the endothelial cell regulates the transendothelial migration of neutrophils during inflammation. Human CD99 has 48% aa sequence identity to mouse CD99.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human CD99 (C-Fc, HEK293)/人CD99蛋白(C-Fc, HEK293) 技术信息
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