货号:A2924353
同义名:
重组人CD47蛋白(C-6His, HEK293)
/ Recombinant Human CD47 (C-6His, HEK293); Leukocyte Surface Antigen CD47
Recombinant Human CD47 (C-6His, HEK293)是人源蛋白,在HEK293人源细胞中表达,带有C端6×His标签。该蛋白是CD47(整合素相关蛋白,IAP),为40–60 kDa的可变糖基化免疫球蛋白超家族成员,广泛表达并参与细胞粘附、钙信号调节及膜运输,与巨噬细胞等免疫细胞的SIRP家族结合,还作为血小板反应蛋白的受体,可能影响免疫逃逸与细胞信号传导。


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| 描述 | CD47(Integrin-Associated Protein,IAP) is a 40 ‑ 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells. CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution, and is reduced in expression on Rh erythrocytes. |
| 蛋白种属 | Human |
| 表达系统 | Human Cells |
| 标签 | C-6His |
| 蛋白表达区间 | Gln19-Pro139 |
| 蛋白编号 | Q08722 |
| 内毒素 | < 1 EU/µg as determined by LAL test. |
| 剂型 | 干粉 |
| 剂型描述 | Lyophilized from a 0.2 μm filtered solution of 10mM Tris-Citrate, 150mM NaCl, pH 8.0. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。 分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
| CAS号 | N/A |
| 分子量 | 14.76 KDa |
| SMILES Code | NONE |
| 别名 | 重组人CD47蛋白(C-6His, HEK293) ;Recombinant Human CD47 (C-6His, HEK293); Leukocyte Surface Antigen CD47; Antigenic Surface Determinant Protein OA3; Integrin-Associated Protein; IAP; Protein MER6; CD47; MER6 |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C: 3-6个月 -80°C: 12个月 Pure form Sealed in dry, store in freezer, under -20°C |
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