Recombinant Human CD40L (N-6His, HEK293)是人源蛋白，在HEK293细胞中表达，带有N端6×His标签。该蛋白为CD40配体，属于TNF超家族II型跨膜糖蛋白，以三聚体形式存在于膜结合及可溶状态，均具生物活性。它能与CD40结合，刺激B细胞增殖及免疫球蛋白分泌，诱导单核细胞产生细胞因子及杀肿瘤活性，并协同激活T细胞增殖，促进IFN-γ、TNF-α和IL-2分泌。
Human CD40L (N-6His, HEK293)/人CD40L蛋白(N-6His, HEK293) 化学结构 CAS号：N/A
Human CD40L (N-6His, HEK293)/人CD40L蛋白(N-6His, HEK293) 3D分子结构 CAS号：N/A
Human CD40L (N-6His, HEK293)/人CD40L蛋白(N-6His, HEK293) 质控信息
Human CD40L (N-6His, HEK293)/人CD40L蛋白(N-6His, HEK293) 生物活性
描述
CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.
Lyophilized from a 0.2 μm filtered solution of PBS, 1mM EDTA, pH 7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human CD40L (N-6His, HEK293)/人CD40L蛋白(N-6His, HEK293) 技术信息
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