Recombinant Human CD3E (C-6His)是人类来源的重组蛋白，在人类细胞中表达，带有C端6×His标签。该蛋白为T细胞表面糖蛋白CD3 ε链（CD3ε），是一种单次跨膜I型蛋白，包含1个免疫球蛋白样结构域和1个ITAM结构域，由位于人类11号染色体上的CD3E基因编码，是T细胞受体-CD3复合体的关键组分，参与T细胞发育及多种胞内信号转导通路。
Human CD3E (C-6His)/人CD3E蛋白(HEK293, C-6His) 化学结构 CAS号：N/A
Human CD3E (C-6His)/人CD3E蛋白(HEK293, C-6His) 3D分子结构 CAS号：N/A
T-Cell Surface Glycoprotein CD3 ε Chain (CD3ε) is a single-pass type I membrane protein. CD3ε contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3ε is a polypeptide encoded by the CD3E gene on chromosome 11 in humans. The T cell receptor-CD3 complex (TCR/CD3 complex) is involved in T-cell development and several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. The T cell receptor-CD3 complex is unique in having ten cytoplasmic immunoreceptor tyrosine-based activation motifs (ITAMs). TCR/CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
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