货号:A2925641
同义名:
重组人CD299蛋白(N-8His-Flag, HEK293)
/ Recombinant Human CD299 (N-8His-Flag, HEK293); CD209L
Recombinant Human CD299 (N-8His-Flag, HEK293) 是人源CD299蛋白,在HEK293细胞中表达,带有N端8His-Flag标签。该蛋白又称DC-SIGNR或CLEC4M,是一种II型跨膜蛋白,可形成同源四聚体,其颈部结构域介导寡聚化。作为病原识别受体,它参与肝脏等外周免疫监视,能以钙依赖方式识别多种含高甘露糖糖链的病毒表面糖蛋白,如HIV、埃博拉病毒、SARS冠状病毒糖蛋白及ICAM3,可能介导病原体内吞与降解。


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| 描述 | CD299 is also known as DC-SIGNR and CLEC4M, is a type II integral membrane protein. DC-SIGNR exists as a homotetramer, and the tandem repeat domain, also called neck domain, mediates oligermerization. Multiple human DC-SIGN/CD209 splice forms exist, generating both membrane-bound and soluble forms. DC-SIGNR is ragarded as a pathogen-recognition receptor involved in peripheral immune surveillance in liver, and probably mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. DC-SIGNR appears to selectively recognize and bind many viral surface glycoproteins containing high mannose N-linked oligosaccharides in a calcium-dependent manner, including HIV-1 gp12, HIV-2 gp12, SIV gp12, ebolavirus glycoproteins, HCV E2, and human SARS coronavirus protein S, as well as the cellular adhesion protein ICAM3. DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation. |
| 蛋白种属 | Human |
| 表达系统 | Human Cells |
| 标签 | N-8His-Flag |
| 蛋白表达区间 | Ser73-Glu376 |
| 蛋白编号 | Q9H2X3-8 |
| 内毒素 | < 1 EU/µg as determined by LAL test. |
| 剂型 | 干粉 |
| 剂型描述 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。 分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
| CAS号 | N/A |
| 分子量 | 37 KDa |
| SMILES Code | NONE |
| 别名 | 重组人CD299蛋白(N-8His-Flag, HEK293) ;Recombinant Human CD299 (N-8His-Flag, HEK293); CD209L; CD209L1; CD299; CLEC4M; C-type lectin domain family 4, member M; DC-SIGN2; DCSIGNR; DC-SIGNR; HP10347; LSIGN; L-SIGN |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C: 3-6个月 -80°C: 12个月 Pure form Sealed in dry, store in freezer, under -20°C |
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