Recombinant Human CD209 (N-Fc, HEK293)是人类来源的重组蛋白，在HEK293人源细胞中表达，带有N端Fc标签。该蛋白又称CD209抗原、DC-SIGN，是一种II型跨膜蛋白，其胞外区为C型凝集素结构域，可结合ICAM-3、病原体表面的甘露糖寡糖及自身糖蛋白，参与树突细胞黏附、迁移、免疫应答启动及病原体和肿瘤的免疫逃逸，具有重要的抗原呈递与免疫调节功能。
Human CD209 (N-Fc, HEK293)/人CD209蛋白(N-Fc, HEK293) 化学结构 CAS号：N/A
Human CD209 (N-Fc, HEK293)/人CD209蛋白(N-Fc, HEK293) 3D分子结构 CAS号：N/A
Human CD209 (N-Fc, HEK293)/人CD209蛋白(N-Fc, HEK293) 质控信息
Human CD209 (N-Fc, HEK293)/人CD209蛋白(N-Fc, HEK293) 生物活性
描述
CD209 is also known as CLEC4L, DC-SIGN and CD209 antigen, is a type II transmembrane protein on DCs with a C-type lectin extracellular domain, is capable of binding ICAM-3 on resting T cells in the secondary lymphoid organs, providing the initial contact between these cells during the establishment of cell-mediated immunity. The DC-SIGN/CD209 lectin domain binds mannose oligosaccharides on pathogens including HIV as well as self glycoproteins including ICAMs (2, 4). DC-SIGN/CD209 binds to butyrophilin 2A1 and this interaction can be blocked by HIV pp120. DC-SIGN/CD209 is expressed on dendritic cells (DC) and inflammatory macrophages and contributes to antigen presentation. It is not only a pattern recognition receptor but implicated in immunoregulation of DCs. It has important role in mediating DC adhesion, migration, inflammation, activating primary T cell, triggering immune response and participating in immune escape of pathogens and tumors.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human CD209 (N-Fc, HEK293)/人CD209蛋白(N-Fc, HEK293) 技术信息
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