Human C5a/人C5a蛋白(E.coli, tag free)
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货号：A2900891同义名：
重组人C5a蛋白(E.coli, tag free)
/ Recombinant Human C5a; Complement C5
Recombinant Human C5a是人补体C5a重组蛋白，来自人源（Human），在大肠杆菌（E.coli）中表达，为无标签（Tag free）蛋白。该蛋白属于过敏毒素家族，是由C5α链经C5a转化酶作用产生的74个氨基酸肽段，具有四段α螺旋及三对链内二硫键形成的三环结构，与人鼠同源性分别为60%和54%。其通过与C5aR/CD88及C5L2受体结合，介导中性粒细胞趋化、内皮细胞活化及炎症介质释放。
Human C5a/人C5a蛋白(E.coli, tag free) 化学结构 CAS号：N/A
Human C5a/人C5a蛋白(E.coli, tag free) 3D分子结构 CAS号：N/A
Human Complement 5a (C5a) is an enzymatically generated glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 74 amino acid (aa) peptide that is created by the activity of C5a convertase on the C5 α-chain. Human C5a has four α-helices plus three intrachain disulfide bonds that create a triple loop structure. Human C5a is 60% and 54% aa identical to mouse and rat C5a, respectively. C5a binds to a signaling G-protein coupled receptor (C5aR/CD88) and a nonsignaling GPCR termed C5L2. Activation of Cd88 results in neutrophil chemotaxis and endothelial cell activation. It also triggers an oxidative burst in macrophages and neutrophils, and induces release of histamine in basophils and mast cells.
Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Human C5a/人C5a蛋白(E.coli, tag free) 细胞实验
Cell Line
Concentration
Treated Time
Description
References
C-fibers
0.1 μg/mL
2 minutes
To evaluate the effect of C5a on ongoing activity of C-fibers, results showed that C5a 0.1 μg/ml did not significantly increase ongoing activity in C-fibers
J Neuroinflammation. 2011 Jul 7;8:80
C-fibers
1 μg/mL
2 minutes
To evaluate the effect of C5a on ongoing activity of C-fibers, results showed that C5a 1 μg/ml increased ongoing activity in 19.0% of C-fibers
J Neuroinflammation. 2011 Jul 7;8:80
RAW264.7 cells
50 ng/mL
24 hours
To investigate the effect of C5a on the expression of TLR4, NLRP3, and CASP1
J Gastroenterol. 2023 Sep;58(9):894-907
Human brain microvascular endothelial cells (HBMVECs)
0.1 µg/mL
48 hours
To evaluate the effect of C5a on apoptosis in HBMVECs, results showed that C5a significantly increased caspase 3/7 activity and DNA damage.
Immunology. 2016 Aug;148(4):407-19
Human C5a/人C5a蛋白(E.coli, tag free) 技术信息
CAS号
N/A
分子量
8.4 KDa
SMILES Code
NONE
别名
重组人C5a蛋白(E.coli, tag free)
;Recombinant Human C5a; Complement C5; C5a anaphylatoxin; C5a
运输
蓝冰
存储条件
In solvent-20°C:3-6个月-80°C:12个月
Pure formSealed in dry, store in freezer, under -20°C
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Tags:
Human C5a/人C5a蛋白(E.coli, tag free)
|Human C5a/人C5a蛋白(E.coli, tag free)
| 重组人C5a蛋白(E.coli, tag free)
| Recombinant Human C5a
| Complement C5
| C5a anaphylatoxin
| C5a
| N/A
| Complement System Proteins
| Human C5a/人C5a蛋白(E.coli, tag free) 纯度/质量文件
| Human C5a/人C5a蛋白(E.coli, tag free) 生物活性
| Human C5a/人C5a蛋白(E.coli, tag free) 技术信息
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