货号:A2900694
同义名:
重组人BGN蛋白(HEK293, C-6His)
/ Recombinant Human BGN (C-6His); Biglycan
Recombinant Human BGN (C-6His)由哺乳动物表达系统生产,目标基因编码Glu20-Lys368,并在C末端带有6His标签。
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描述 | Biglycan is a 200-350 kD proteoglycan consisting of a 45 kD core protein and two chrondroitin/dermatan sulfate glycosaminoglycan chains. Biglycan binds to TGF-ß. It also binds to collagen type I in low ionic strength (less than 3 mM phosphate) buffer. At higher ionic strengths, Biglycan does not bind to collagen type I. It enhances the inhibition effect of TGF-ß on osteoclast proliferation at a concentration of 4-20 mg/ml. It also prevents the attachment of CHO cells to fibronectin, with a 50% inhibition at 17-21 mg/ml. |
蛋白种属 | Human |
表达系统 | Human Cells |
标签 | C-6His |
蛋白表达区间 | Glu20-Lys368 |
蛋白编号 | P21810 |
内毒素 | < 1 EU/µg as determined by LAL test. |
剂型 | 干粉 |
剂型描述 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
CAS号 | N/A |
分子量 | 40.47 KDa |
SMILES Code | NONE |
别名 | 重组人BGN蛋白(HEK293, C-6His) ;Recombinant Human BGN (C-6His); Biglycan; Bone/Cartilage Proteoglycan I; PG-S1; BGN; SLRR1A |
运输 | 蓝冰 |
存储条件 |
In solvent -20°C: 3-6个月 -80°C: 12个月 Pure form Sealed in dry, store in freezer, under -20°C |