Glycitin, a natural isoflavone, could promote the proliferation of bone marrow stromal cells and osteoblasts and protect the loss of bone. It can be isolated and purified from legumes. Administration of glycitin increased cell proliferation and promoted osteoblast formation from BMSCs. Furthermore, glycitin activated the gene expression of Col I and ALP in BMSCs. Notably, glycitin suppressed protein expression of TGF-β and AKT in BMSCs[3]. Co-treatment with TMF and glycitin synergistically promotes the proliferation and migration of both keratinocytes and dermal fibroblasts, with a 1:1 ratio of these compounds showing the greatest efficacy in our co-culture system[4]. Glycitin significantly prevents bone loss in variectomized (ovx) rats at a dose of 50 mg/kg/d. At this dose glycitin also prevents ovx-induced uterine atrophy and increases in body weight gain, abdominal fat, serum total cholesterol and triglyceride, and urinary excretion of pyridinoline and deoxypyridinoline with statistical significance[5].
Glycitin/黄豆黄苷 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Human dermal fibroblasts
10 μM
24 hours
Promote proliferation and migration of fibroblasts
Exp Mol Med. 2017 Mar 17;49(3):e302
HaCaT keratinocytes
10 μM
24 hours
Promote proliferation and migration of keratinocytes
Exp Mol Med. 2017 Mar 17;49(3):e302
THP-1
25, 50, 100, 200, 400 μM
48 hours
Evaluate the effect of Glycitin on cell viability, results showed concentrations below 50 μM were non-toxic to the cells
Cell Biol Toxicol. 2025 Feb 8;41(1):41
H1299
25, 50, 100, 200, 400 μM
48 hours
Evaluate the effect of Glycitin on cell viability, results showed concentrations below 50 μM were non-toxic to the cells
Cell Biol Toxicol. 2025 Feb 8;41(1):41
A549
25, 50, 100, 200, 400 μM
48 hours
Evaluate the effect of Glycitin on cell viability, results showed concentrations below 50 μM were non-toxic to the cells
40 µW/cm² UV-B radiation for 2 h, followed by 12 h incubation in the dark
UV-B radiation significantly increased Glycitin content in cotyledon suspension cultures
Foods. 2024 Jul 28;13(15):2385
Glycitin/黄豆黄苷 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
ICR mice
Excisional wound model
Topical administration
200 μM
Once daily for 14 days
Promote wound closure and reduce scar formation
Exp Mol Med. 2017 Mar 17;49(3):e302
Male Wistar rats
Pentylenetetrazol (PTZ)-induced seizure model
Intraperitoneal injection
5, 10, and 20 mg/kg
Once every 48 hours for a total of 21 days
To evaluate the anticonvulsant effect of Glycitin on PTZ-induced seizures and its impact on oxidative stress and the Nrf2/HO-1 signaling pathway. Results showed that Glycitin pretreatment effectively mitigated PTZ-induced convulsive behaviors and improved oxidative stress markers (MDA, GPx, and SOD levels), while activating the Nrf2/HO-1 signaling pathway.
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