DNA polymerases are essential enzymes responsible for replication and repair of DNA in all organisms[3]. Foscarnet Sodium is a viral DNA polymerase activity inhibitor,Foscarnet sodium can inhibit dNTPs thus attenuats viral RNA polymerases, reverse transcriptase, and DNA polymerases. It is used in the treatment of cytomegalovirus retinitis[4].The simultaneous infusion of foscarnet increased the renal clearance of lithium and its ratio to creatinine clearance as well as the urinary excretion of phosphate. Foscarnet also inhibited the lithium-induced phosphorylation of GSK3β[5].Foscarnet in phosphate-buffered saline decreased in a dose-dependent manner the viability of cultured human skin fibroblasts. This toxic effect was markedly decreased when foscarnet was incorporated into the polymer matrix[6].Foscarnet competes with ATP against the binding on fibroblast growth factor 2 at the heparin/ATP-binding domain. This indicates binding of foscarnet at the heparin-binding domain of FGF2. This interaction of foscarnet with fibroblast growth factor 2 reduces FGF2-induced proliferation of human umbilical vein endothelial cells and intracellular signaling via ERK1/2 kinases in this cell line[7].
Foscarnet sodium/膦甲酸钠 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Klebsiella pneumoniae Kp142
0.623 mM
24 hours
Evaluate the synergistic effect of PPF with fosfomycin, showing that PPF at 0.623 mM concentration has a synergistic effect with fosfomycin.
Antimicrob Agents Chemother. 2021 Feb 17;65(3):e01911-20
Klebsiella pneumoniae Kp12
0.623 mM
24 hours
Evaluate the synergistic effect of PPF with fosfomycin, showing that PPF at 0.623 mM concentration has a synergistic effect with fosfomycin.
Antimicrob Agents Chemother. 2021 Feb 17;65(3):e01911-20
Klebsiella pneumoniae ATCC 700721
0.623 mM
24 hours
Evaluate the synergistic effect of PPF with fosfomycin, showing that PPF at 0.623 mM concentration has a synergistic effect with fosfomycin.
Antimicrob Agents Chemother. 2021 Feb 17;65(3):e01911-20
RPE-1 cells
200 µM
72 hours
To evaluate the inhibitory effect of foscarnet on HCMV gene expression, results showed that foscarnet dampened mCherry-SCP expression and the second phase of mNeonGreen-ie1/2
Front Cell Infect Microbiol. 2021 Jan 8;10:536150
MRC-5 cells
200 µM
72 hours
To evaluate the inhibitory effect of foscarnet on HCMV gene expression, results showed that foscarnet dampened mCherry-SCP expression and the second phase of mNeonGreen-ie1/2
Front Cell Infect Microbiol. 2021 Jan 8;10:536150
MRC-5 cells
0 to 400 μM (WT), 0 to 100 μM (hypersusceptible viruses)
6 days
Evaluate the susceptibility of recombinant HCMV viruses to foscarnet sodium, showing hypersusceptibility of Q579I and K805Q mutant viruses (EC50 0.12- and 0.19-fold that of WT, respectively)
Antimicrob Agents Chemother. 2020 Mar 24;64(4):e01910-19
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