Erianin directly bound to NLRP3, inhibiting its ATPase activity and thereby blocking NLRP3 inflammasome assembly.
Front Immunol. 2021 Oct 20;12:739953.
THP-1 cells
5 nM
6 hours
Erianin suppressed NLRP3 inflammasome activation by preventing NEK7-NLRP3 and ASC-NLRP3 interactions.
Front Immunol. 2021 Oct 20;12:739953.
HCT116 (KRAS mutant)
12.5, 25, 50 nM
24 hours
Inhibited constitutive activation of MAPK signaling pathway
Signal Transduct Target Ther. 2023 Mar 6;8(1):96.
A375 (BRAF V600E mutant)
12.5, 25, 50 nM
24 hours
Inhibited constitutive activation of MAPK signaling pathway
Signal Transduct Target Ther. 2023 Mar 6;8(1):96.
SK-MEL-2 (NRAS mutant)
12.5, 25, 50 nM
24 hours
Inhibited constitutive activation of MAPK signaling pathway
Signal Transduct Target Ther. 2023 Mar 6;8(1):96.
MG63.2 cells
0, 10, 25, 50, 100 nM
24, 48, 72 hours
Erianin inhibited the proliferation of MG63.2 cells in a time and dose-dependent manner with IC50 values of 88.69 nM (24 h), 44.26 nM (48 h), and 17.20 nM (72 h).
Cell Death Dis. 2016 Jun 2;7(6):e2247.
143B cells
0, 10, 25, 50, 100 nM
24, 48, 72 hours
Erianin inhibited the proliferation of 143B cells in a time and dose-dependent manner with IC50 values of 58.19 nM (24 h), 40.97 nM (48 h), and 26.77 nM (72 h).
Cell Death Dis. 2016 Jun 2;7(6):e2247.
NCI-H1299 (H1299)
200, 100, 50, 25, 12.5, and 0 nM
24 hours
To evaluate the effect of Erianin on lung cancer cell viability. Erianin significantly reduced the survival rate of both cell lines, with IC50 values of 61.33 nM for H460 cells and 21.89 nM for H1299 cells.
Cancer Biol Med. 2025 Feb 24;22(2):144–65.
NCI-H460 (H460)
200, 100, 50, 25, 12.5, and 0 nM
24 hours
To evaluate the effect of Erianin on lung cancer cell viability. Erianin significantly reduced the survival rate of both cell lines, with IC50 values of 61.33 nM for H460 cells and 21.89 nM for H1299 cells.
Cancer Biol Med. 2025 Feb 24;22(2):144–65.
Human immortalized keratinocyte (HaCaT)
30 nM
24 hours
To evaluate the anti-proliferative and pro-apoptotic effects of E/DMSNs on HaCaT cells. Results showed that E/DMSNs had stronger anti-proliferative and pro-apoptotic effects than free erianin.
J Nanobiotechnology. 2020 Mar 30;18(1):55.
Human Retinal Vascular Endothelial Cells (HRVECs)
10-100 nM
24 hours
Erianin significantly inhibited the proliferation, migration, tube formation, and adhesion of hypoxia-induced HRVECs in a concentration-dependent manner. The inhibitory effect on cell proliferation and tube formation was more pronounced in the collagen-cultured group.
Invest Ophthalmol Vis Sci. 2022 Jan 3;63(1):27.
Erianin/毛兰素 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
C57BL/6 mice
MSU-induced peritonitis and gouty arthritis models
Intraperitoneal injection
5 mg/kg
Single injection, lasting 12 hours
Erianin exhibited dose-dependent inhibitory effects on MSU-induced IL-1β and IL-18 production and neutrophil migration but had no effect on TNF-α production.
Front Immunol. 2021 Oct 20;12:739953.
NOD-SCID mice
A375 (BRAF V600E mutant) and SK-MEL-2 (NRAS mutant) cell-derived xenograft model
Oral gavage
50 mg/kg
Once daily, tumor volume was monitored twice per week
Inhibited tumor growth and reduced tumor volume and weight
Signal Transduct Target Ther. 2023 Mar 6;8(1):96.
BALB/c nude mice
H460 cell xenograft model
Intraperitoneal injection
100 mg/kg
Once daily for 15 days
Erianin notably suppressed tumor growth
Signal Transduct Target Ther. 2020 May 8;5(1):51
BALB/c-nu mice
Osteosarcoma xenograft model
Intraperitoneal injection
2 mg/kg
Every other day for seven times
Erianin significantly inhibited tumor growth with little organ-related toxicity.
Cell Death Dis. 2016 Jun 2;7(6):e2247.
Nude mice
Lung cancer-bearing nude mice model
Intraperitoneal injection
50 mg/kg, 100 mg/kg, and 200 mg/kg
Every 2nd day for 18 days
To evaluate the inhibitory effects of Erianin on lung cancer growth in vivo. Medium and high doses of Erianin significantly inhibited tumor growth without significant effects on body weight.
Cancer Biol Med. 2025 Feb 24;22(2):144–65.
Zebrafish (adult and embryos)
Hypoxia-induced retinopathy model
Embryo medium (embryo model) and intravitreal injection (adult model)
100-1000 nM
Embryo model: 4 days; Adult model: 7 days
Erianin significantly reduced hypoxia-induced retinal angiogenesis in zebrafish in a concentration-dependent manner. In the adult model, the inhibitory effects on vascular branch points, sprouts, and vascularization areas were more pronounced.
Invest Ophthalmol Vis Sci. 2022 Jan 3;63(1):27.
Erianin/毛兰素 实验方案
计算器
存储液制备
1mg
5mg
10mg
1 mM
5 mM
10 mM
3.14mL
0.63mL
0.31mL
15.71mL
3.14mL
1.57mL
31.41mL
6.28mL
3.14mL
Erianin/毛兰素 技术信息
CAS号
95041-90-0
分子式
C18H22O5
分子量
318.36
SMILES Code
COC1=C(C=C(C=C1)CCC2=CC(OC)=C(C(OC)=C2)OC)O
MDL No.
MFCD06795132
别名
NSC 613744
运输
蓝冰
InChI Key
UXDFUVFNIAJEGM-UHFFFAOYSA-N
Pubchem ID
356759
存储条件
In solvent-20°C:3-6个月-80°C:12个月
Pure formKeep in dark place,Inert atmosphere,2-8°C
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