EGCG significantly inhibited iNOS protein expression and supernatant NO levels, and reduced IL-6 production.
Cell Mol Immunol. 2010 Mar;7(2):123-32.
Naïve CD4+ T cells
10 μM
72 h
To evaluate the effect of EGCG on the proliferation of naïve CD4+ T cells. EGCG treatment inhibited the division of naïve CD4+ T cells stimulated with anti-CD3/CD28 antibodies, increasing the proportion of cells arrested in the G0/G1 phase by 8.6% and reducing DNA synthesis activity in the S phase by 51%.
Curr Res Food Sci. 2023 Jun 18;7:100537.
Human umbilical vein endothelial cells (HUVECS)
100 µM
60 min
EGCG increased NO levels and restored NO levels in PE plasma-treated cells via the PI3K/Akt/eNOS pathway.
Antioxidants (Basel). 2024 Jan 26;13(2):158.
Primary human hepatocytes (PHHs)
12.5, 25, and 50 μM
24 h
EGCG significantly downregulated HMGB1 secretion and cytoplasmic HMGB1 levels in HBV-infected PHHs
Front Immunol. 2023 Apr 14;14:1147379.
HepAD38 cells
12.5, 25, and 50 μM
24 h
EGCG significantly downregulated HMGB1 secretion in HBV-replicating hepatoma cells and activated autophagic degradation of cytoplasmic HMGB1
EGCG treatment promoted the viability of OGD/R cells and suppressed LDH levels, with 40 μM EGCG showing the best effects.
Chin Med. 2023 Dec 6;18(1):160.
HL-1 cells
0, 5, 10, 20, 40, 80, 100 μM
3 h
To evaluate the effect of EGCG on HL-1 cell viability, results showed that EGCG treatment did not exert significant toxicity on HL-1 cells.
Chin Med. 2023 Dec 6;18(1):160.
EGCG Octaacetate 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Caenorhabditis elegans
Wild-type N2 nematodes
Smeared on the surface of agar
200 μM and 1000 μM
Survival was recorded every two days from the first day of adulthood until death.
To study the alleviating effect of theanine on high-dose EGCG-induced lifespan reduction. Results showed that theanine could alleviate the shortened lifespan induced by high-dose EGCG and improve locomotor ability and body length reduction.
Foods. 2021 Jun 17;10(6):1404.
Sprague-Dawley rats
Natural aging rats with cognitive impairments
Intragastric administration
100 mg/kg
Once daily for 4 weeks
EGCG significantly ameliorated learning and memory impairments in natural aging rats with CI and decreased Aβ1–42 plaque formation in their brains
Acta Pharmacol Sin. 2019 Nov;40(11):1490-1500
Sprague-Dawley rats
Lithium-pilocarpine-induced status epilepticus model
Intracerebroventricular injection
50 μM
Continuous administration
EGCG attenuates CA1 neuronal death induced by status epilepticus by regulating GPx1, NF-κB S536 phosphorylation, and mitochondrial dynamics
Antioxidants (Basel). 2023 Apr 20;12(4):966
Mice
Recombinant cccDNA (rcccDNA) chronic HBV mouse model
Intraperitoneal injection
25 mg/kg
Daily for 42 consecutive days
EGCG treatment significantly alleviated HBV-induced liver injury and fibrosis by autophagic degradation of HMGB1 in hepatocytes and suppression of macrophage NLRP3 inflammasome activation
EGCG significantly reduced cigarette smoke-induced benzopyrene-DNA adduct formation and the development of precancerous bronchial epithelial lesions.
Int J Mol Med. 2021 Dec;48(6):220.
C57BL mice
Myocardial infarction model
Oral gavage
50 mg/kg
Once daily for 4 weeks
EGCG treatment improved cardiac function in MI mice, reduced myocardial infarction size, and inhibited AIM2 inflammasome-mediated pyroptosis.
Chin Med. 2023 Dec 6;18(1):160.
Sprague-Dawley rats
Cardiac arrest/cardiopulmonary resuscitation (CA/CPR) model
Intravenous injection
3 mg/kg and 9 mg/kg
Single dose, observed for 24 hours
EGCG dose-dependently alleviated the IRI after CA/CPR, inhibited ERK activity and restored mitochondrial function, as indicated by improved NDS, reduced ROS level, decreased mPTP opening, elevated ATP content, increased ATPase expression and downregulated cleaved-caspase 3 level.
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