Dynarrestin {[allProObj[0].p_purity_real_show]}

Dynarrestin 质控信息

Dynarrestin 生物活性

描述

Dynarrestin is an aminothiazole inhibitor of cytoplasmic dyneins 1 and 2. Dynarrestin rapidly and reversibly inhibits microtubule gliding driven by dyneins 1 in vitro, as well as a range of intracellular processes dependent on dyneins 1 and 2, without affecting ATP hydrolysis or interfering with ciliogenesis. Dynarrestin inhibits Hh-dependent proliferation of neuronal precursors and tumour cells[1].

Dynarrestin 细胞实验

Cell Line NIH3T3 cells IMCD3 cells Cos7 cells mouse hippocampal neurons	Concentration	Treated Time	Description	References
NIH3T3 cells	1 μM	8 hours	Evaluate Smo trafficking, found Smo accumulated in cilia	Cell Chem Biol. 2018 Apr 19;25(4):357-369. e6
IMCD3 cells	25 μM		Evaluate endomembrane translocation, found movement was profoundly inhibited	Cell Chem Biol. 2018 Apr 19;25(4):357-369. e6
Cos7 cells	25 μM	60 minutes	Evaluate endomembrane translocation, found long-range, bidirectional movement was profoundly inhibited	Cell Chem Biol. 2018 Apr 19;25(4):357-369. e6
mouse hippocampal neurons	100 μM or 200 μM	30 minutes	Investigated the effect of dynarrestin on synaptic cargo transport, finding that dynarrestin decreased the FPU for retrograde transport more than for anterograde transport.	Biophys J. 2021 May 4;120(9):1605-1614
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Dynarrestin 动物实验

Species Mouse	Animal Model Ptch−/+ medulloblastoma model	Administration	Dosage	Frequency	Description	References
Mouse	Ptch−/+ medulloblastoma model	In vitro culture	0.35 μM		Evaluate tumor cell proliferation inhibition, found Dynarrestin inhibits Hh-dependent tumor cell proliferation	Cell Chem Biol. 2018 Apr 19;25(4):357-369. e6
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Dynarrestin 参考文献

Dynarrestin 实验方案

计算器	摩尔计算器 总药量计算器 工作液计算器
存储液制备		1mg	5mg	10mg
	1 mM 5 mM 10 mM	2.32mL 0.46mL 0.23mL	11.59mL 2.32mL 1.16mL	23.17mL 4.63mL 2.32mL

Dynarrestin 技术信息