Doxycycline hyclate is a common broad-spectrum antibiotic. It is also an inhibitor of MMP-8 (nuclear collagenase; IC50 = 30 μM). It also has a certain inhibitory effect on MMP-1 (collagenase-1, IC50 = 300 μM). Its adequate oral absorption and extended half-life, which allows fewer daily doses. Doxycycline hyclate is considered the tetracycline of choice in patients with poor renal function due to its limited clearance by kidneys. It is active against protozoa and can be administered in combination with quinine in the management of chloroquine resistant plasmodium falciparum infection[3].
Doxycycline hyclate/盐酸强力霉素 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HeLa cells
5 ng/ml
Induce the expression of hSSB1-WT or hSSB1-DM
Signal Transduct Target Ther. 2020 Jun 24;5(1):80.
H1 cells
1 μM
2 or 3 days
To assess the function of SMARCB1 in steady state hESCs, qPCR and western blot results showed strong downregulation of SMARCB1 at the transcript and protein levels
Elife. 2019 Apr 29;8:e45672.
A375VR4
1 µg/mL
5–6 days
To induce shRNA expression for studying the effects of MTH1 knockdown.
Cell Death Differ. 2020 Jul;27(7):2081-2098.
human fibroblasts
100 ng/mL
24 h
To investigate the effect of Doxycycline-induced myr-Akt expression on HCMV replication, results showed that myr-Akt expression caused a roughly 10-fold decrease in viral replication.
mBio. 2022 Aug 30;13(4):e0104222.
HMLE-Twist-ER cells
1–2 μg/mL
15 days
Induced LRIG1 expression to limit the expression of EMT markers. Results showed that LRIG1 expression blunted the expression of Fibronectin, Slug, and CD44, indicating that LRIG1 restricts the full expression of EMT markers.
Oncogene. 2016 Jun 2;35(22):2932-47.
Doxycycline hyclate/盐酸强力霉素 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Nude mice
H1299 xenograft model
Oral
625 mg/kg
Daily for 4 weeks
To investigate the effect of SAT1 overexpression on tumor growth, results showed that SAT1 induction significantly suppressed xenograft tumor growth.
Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):E6806-E6812
Nude mice
HCT116 xenograft model
Intraperitoneal injection
5 mg/kg
Every 2 days for 10 days
Evaluate the effect of combined treatment of 2-D08 and etoposide on tumor growth
Signal Transduct Target Ther. 2020 Jun 24;5(1):80.
Mice
TGFα-induced pulmonary fibrosis model
Oral
62.5 mg/kg
6 weeks
Induced overexpression of TGFα by feeding mice with doxycycline-containing chow to establish a pulmonary fibrosis model.
EMBO Mol Med. 2020 Sep 7;12(9):e12131
Mice
Cutaneous squamous cell carcinoma (SCC) model
Oral
200mg
Continuous administration
To induce RNAi-mediated knockdown of the SOX2 gene to study its role in cutaneous squamous cell carcinoma growth. Results showed that SOX2 knockdown significantly reduced tumor growth.
Nat Commun. 2014 Jul 31;5:4511
Mice
ATG5i mouse model
Dietary administration
200 ppm
6 weeks
To evaluate the effects of systemic Atg5 knockdown, ATG5i mice exhibited many phenotypes similar to tissue-specific knockout models, such as hepatomegaly, reduced adipose tissue, and pancreatic degeneration
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