Microtubules are the main constituent of mitotic apparatus in all eukaryotic cells, thus make it become an important pharmacological target for the treatment of tumor . Docetaxel Trihydrate is the trihydrate form of Docetaxel. Docetaxel, belong to the taxane family including paclitaxel, can promote the assembly and polymerization of microtubule with an appearance of twice as active in depolymerization inhibition compared with paclitaxel . When treated with Docetaxel for 72h, the significant growth inhibition can be observed in ovarian cancer cell line, including A2780, H134, IGROV-1 and OVCAR-3, with IC50 ranging from 2.8-87nM. Activation of caspase, upregulation of Bcl-2 phosphorylation and apoptosis can be observed in cells with prolonged G2/M arrest by Docetaxel, which may be one of the mechanism for cell death induced by Docetaxel in tumor therapy. However, Docetaxel also shows antiangiogenic effect in in vitro and in vivo studies. It can inhibit HUVEC proliferation in a dose-dependent manner with an IC50 of 0.27 ng/ml, as well as reduce 54% of angiogenesis level compared with control on dose of 3 mg/kg in MCF-7 tumor bearing nude mice .Docetaxel is approved by FDA for treatment of head and neck cancer, gastric cancer, breast cancer, prostate cancer and non-small cell lung cancer (see in FDA net).
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