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Diethylstilbestrol/己烯雌酚 {[allProObj[0].p_purity_real_show]}

货号:A174248 同义名: Stilbestrol

Diethylstilbestrol 是一种合成非类固醇雌激素,产生与天然雌二醇相似的药理学效应,常用于雌激素缺乏及激素失衡相关研究。

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Type HazMat fee for 500 gram (Estimated)
Excepted Quantity USD 0.00
Limited Quantity USD 15-60
Inaccessible (Haz class 6.1), Domestic USD 80+
Inaccessible (Haz class 6.1), International USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic USD 100+
Accessible (Haz class 3, 4, 5 or 8), International USD 200+
Diethylstilbestrol/己烯雌酚 化学结构 CAS号:56-53-1
Diethylstilbestrol/己烯雌酚 化学结构
CAS号:56-53-1
Diethylstilbestrol/己烯雌酚 3D分子结构
CAS号:56-53-1
Diethylstilbestrol/己烯雌酚 化学结构 CAS号:56-53-1
Diethylstilbestrol/己烯雌酚 3D分子结构 CAS号:56-53-1
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Diethylstilbestrol/己烯雌酚 纯度/质量文件 产品仅供科研

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Diethylstilbestrol/己烯雌酚 生物活性

描述 Diethylstilbestrol is a synthetic estrogen receptor agonist structurally related to estradiol. Diethylstilbestrol at 20µM results in 30±13% and 43±4% displacement of [3H]-estradiol from the sex hormone-binding globulin steroid-binding site in the serum and dextran-coated charcoal-treated serum[3]. Prostate cancer PC-3 cells treated with 30µM diethylstilbestrol for 72 hours show smaller nuclei compared with the control group. Diethylstilbestrol at 15 and 30µM induces apoptosis of androgen-insensitive prostate cancer cells[4].

Diethylstilbestrol/己烯雌酚 细胞实验

Cell Line
Concentration Treated Time Description References
Human umbilical vein endothelial cells (HUVECs) 25-100 μM DES significantly reduced HUVECs viability, inhibited VEGF-mediated wound closure and migration, and diminished capillary-like tube formation. Clin Transl Med. 2020 Jun;10(2):e47.
embryonic thymocytes 25 μM 18-24 h To investigate the effects of DES on embryonic thymocyte death and differentiation, results showed DES induced cell death. Int J Mol Sci. 2021 Sep 20;22(18):10138.
embryonic thymocytes 50 μM 18-24 h To investigate the effects of DES on embryonic thymocyte death and differentiation, results showed DES induced cell death. Int J Mol Sci. 2021 Sep 20;22(18):10138.
chicken oviductal epithelial cells (cOECs) 100 μM 1 week Upregulated oviduct-specific genes (OVA, OVM, LYZ, AVD, ESR1) and enhanced secretion of EGFP and OVA proteins Poult Sci. 2024 Jun;103(6):103723.

Diethylstilbestrol/己烯雌酚 动物实验

Species
Animal Model
Administration Dosage Frequency Description References
Zebrafish Juvenile zebrafish Water exposure 100 and 1000 ng/L From 10 to 90 dpf, exposed in three stages (initial development stage (IDS, 10-25 dpf), gonadal differentiation stage (GDS, 25-45 dpf), and gonadal maturity stage (GMS, 45-60 dpf)) To investigate the effects of DES on zebrafish gonad development and endocrine disruption mechanism at different developmental stages. Results showed that DES exposure during the gonadal differentiation stage (GDS) significantly reduced growth indicators (body length,, etc.) and significantly increased the proportion of females (59.65%), indicating a bias towards female differentiation. Biomolecules. 2021 Jun 25;11(7):941
Mice Male mouse model Oral 0.2 µg/kg Once daily from GD 11 to 17 and from PND 1 to 15 To investigate the effects of perinatal DES exposure on the adult male urogenital tract, results showed DES exposure led to enlarged prostates, enlarged bladders, and urethral obstruction. Int J Mol Sci. 2020 May 30;21(11):3902

Diethylstilbestrol/己烯雌酚 临床研究

NCT号 适应症或疾病 临床期 招募状态 预计完成时间 地点
NCT00428948 Polycystic Kidney Disease, Aut... 展开 >>osomal Dominant 收起 << Phase 3 Completed - -
NCT00428948 - Completed - -
NCT01212991 Prostate Cancer Phase 3 Active, not recruiting December 28, 2018 -

Diethylstilbestrol/己烯雌酚 参考文献

[1]Tremblay GB, Kunath T, et al. Diethylstilbestrol regulates trophoblast stem cell differentiation as a ligand of orphan nuclear receptor ERR beta. Genes Dev. 2001 Apr 1;15(7):833-8.

[2]Sharpe RM, Atanassova N, et al. Abnormalities in functional development of the Sertoli cells in rats treated neonatally with diethylstilbestrol: a possible role for estrogens in Sertoli cell development. Biol Reprod. 1998 Nov;59(5):1084-94.

[3]Hodgert Jury H, Zacharewski TR, Hammond GL. Interactions between human plasma sex hormone-binding globulin and xenobiotic ligands. J Steroid Biochem Mol Biol. 2000;75(2-3):167-176.

[4]Robertson CN, Roberson KM, Padilla GM, et al. Induction of apoptosis by diethylstilbestrol in hormone-insensitive prostate cancer cells. J Natl Cancer Inst. 1996;88(13):908-917.

Diethylstilbestrol/己烯雌酚 实验方案

计算器
存储液制备 1mg 5mg 10mg

1 mM

5 mM

10 mM

3.73mL

0.75mL

0.37mL

18.63mL

3.73mL

1.86mL

37.26mL

7.45mL

3.73mL

Diethylstilbestrol/己烯雌酚 技术信息

CAS号56-53-1
分子式C18H20O2
分子量 268.35
SMILES Code OC1=CC=C(C=C1)/C(CC)=C(CC)/C2=CC=C(O)C=C2
MDL No. MFCD00002373
别名 Stilbestrol
运输蓝冰
InChI Key RGLYKWWBQGJZGM-ISLYRVAYSA-N
Pubchem ID 448537
存储条件

In solvent -20°C: 3-6个月 -80°C: 12个月

Pure form Sealed in dry, room temperature

溶解方案

DMSO: 50 mg/mL(186.32 mM),配合低频超声助溶,注意:DMSO长时间开封后,会吸水并导致溶解能力下降,请避免使用长期开封的DMSO

请根据您的动物给药指南选择适当的溶解方案。
以下溶解方案都请先按照体外实验的方式配制澄清的储备液,再依次添加助溶剂:
——为保证实验结果的可靠性,澄清的储备液可以根据储存条件,适当保存;体内实验的工作液,建议现用现配,当天使用; 以下溶剂前显示的百分比是指该溶剂在终溶液中的体积占比;如在配制过程中出现沉淀、析出现象,可以通过加热和/或超声的方式助溶
方案 一
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