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Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
Coptisine is a protoberberine isoquinoline alkaloid isolated and purified from the herbs of Chelidonium majus L., which can increases cell viability based on its reversal effect on the enhanced activity of Indoleamine 2,3-dioxygenase .
Coptisine/黄连碱 细胞实验
Cell Line
Concentration
Treated Time
Description
References
NCI-H1299 cells
10 μM or 20 μM
24 h
COP significantly reduced the expression of ATF4 induced by glutamine restriction, leading to substantial inhibition of cancer cell proliferation.
Adv Sci (Weinh). 2024 Sep;11(35):e2401748
NCI-H460 cells
10 μM or 20 μM
24 h
COP significantly reduced the expression of ATF4 induced by glutamine restriction, leading to substantial inhibition of cancer cell proliferation.
Adv Sci (Weinh). 2024 Sep;11(35):e2401748
Helicobacter pylori clinical isolates
25-50 μg/mL
3 days
To evaluate the antibacterial activity of Coptisine against clinical isolates, results showed that the MICs of Coptisine against clinical isolates were either 25 or 50 μg/mL
J Enzyme Inhib Med Chem. 2018 Dec;33(1):1362-1375
Helicobacter pylori standard strain NCTC 11637
25 μg/mL
3 days
To evaluate the antibacterial activity of Coptisine against H. pylori, results showed that the MIC of Coptisine against NCTC 11637 strain was 25 μg/mL
J Enzyme Inhib Med Chem. 2018 Dec;33(1):1362-1375
Hep3B cells
50 µM
24 h
Coptisine markedly increased DNA damage and apoptotic cell death, which was associated with induction of death receptor proteins. Coptisine also significantly upregulated expression of proapoptotic Bax protein, downregulated expression of anti-apoptotic Bcl-2 protein, and activated caspase-3, -8, and -9. In addition, coptisine remarkably increased the generation of reactive oxygen species (ROS), loss of mitochondrial membrane potential (MMP), and release of cytochrome c into the cytoplasm.
Int J Mol Sci. 2020 Jul 31;21(15):5502
MOLM-13 cells
0–20 μM
12 h
To evaluate the inhibitory activity of Coptisine on METTL3 and its effect on m6A modification levels. Results showed that Coptisine dose-dependently decreased m6A levels of mRNAs.
Adv Sci (Weinh). 2024 Jul;11(26):e2308786
BEAS-2B
10 μM
24 h
no significant effect on KRAS mRNA levels
Nat Commun. 2022 Oct 12;13(1):6016
A549
10 μM
24 h
significantly lowered KRAS mRNA levels
Nat Commun. 2022 Oct 12;13(1):6016
H460
10 μM
24 h
significantly lowered KRAS mRNA levels
Nat Commun. 2022 Oct 12;13(1):6016
Coptisine/黄连碱 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
BALB/c nude mice
H460 xenograft tumor model
Intraperitoneal injection
100 mg/kg
Every other day for two weeks
COP significantly enhanced the inhibitory effect of glutamine-restrictive therapy on tumor growth and effectively disrupted the GCN2-ATF4-ASCT2 axis.
Adv Sci (Weinh). 2024 Sep;11(35):e2401748
Mice
Periodontitis models
Local injection
20 μM
Daily, until the experimental endpoint
To evaluate the therapeutic effect of Coptisine on periodontitis. Results showed that Coptisine significantly alleviated inflammatory periodontal bone loss and reduced the expression of IL-1β and GSDMD.
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