Calycosin-7-O-β-D-glucoside, a melanin biosynthesis inhibitor, is isolated from the methanol extract of astragalus.
Calycosin-7-O-β-D-glucoside/毛蕊异黄酮苷 细胞实验
Cell Line
Concentration
Treated Time
Description
References
Human mesenchymal stem cells
1-10 µM
21 days
Evaluate late osteogenic differentiation, results showed that Caly promoted the formation of mineralized nodules
Int J Mol Sci. 2021 Oct 21;22(21):11362.
Human mesenchymal stem cells
1-10 µM
24 hours
Evaluate cell migration ability, results showed that Caly significantly promoted the migration of MSCs
Int J Mol Sci. 2021 Oct 21;22(21):11362.
NHBE cells
1000 µg/ml and 500 µg/ml
24 hours
To evaluate the effect of AR on CSE-induced autophagy in NHBE cells, results showed that AR significantly reduced the number of autophagosomes and autolysosomes and increased cellular structural integrity.
Front Pharmacol. 2021 Jan 14;11:580978.
HT22 cells
35, 70, 140 µM
24 hours
To investigate the protective effect of CAG on OGD/R-injured HT22 cells and its potential mechanism. Results showed that CAG significantly reduced ROS levels, apoptosis rate, and improved cell viability.
Molecules. 2025 Jan 25;30(3):549.
B16F10 cells
1–1000 µg/mL
48 hours
To evaluate the effect of YQD on melanogenesis in B16F10 cells. Results showed that YQD increased melanin content in B16F10 cells in a dose-dependent manner, significantly enhanced cellular tyrosinase activity, and upregulated TRP-1 gene and protein expression.
Pharmaceuticals (Basel). 2023 Dec 11;16(12):1716.
Mouse liver microsomes
100 ng/mL
60 minutes
To study the effect of CYP450 enzyme-specific inhibitors on the metabolism of Calycosin-7-O-β-D-glucoside, results showed no significant effect.
Front Pharmacol. 2023 Apr 14;14:1175896.
Human mesenchymal stem cells
1-10 µM
7 days
Evaluate early osteogenic differentiation, results showed that Caly increased ALP expression and activity
Int J Mol Sci. 2021 Oct 21;22(21):11362.
Calycosin-7-O-β-D-glucoside/毛蕊异黄酮苷 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Sprague-Dawley (SD) rats
Rheumatoid arthritis model
Oral gavage
0.648 g/100g/d
7 consecutive days
To evaluate the therapeutic effect of Xinfeng Capsule on rheumatoid arthritis. Results showed that Xinfeng Capsule significantly improved joint histopathological damage, inhibited NF-κB pathway activation, and promoted synovial cell apoptosis.
Front Immunol. 2025 Mar 13;16:1556764
Mice
Diabetic nephropathy model
Oral
0.96 g/kg, 0.48 g/kg, and 0.24 g/kg
Once daily for 12 weeks
To investigate the effects of HQD on diabetic nephropathy and the tissue distribution difference of its active constituents between normal and diabetic nephropathy mouse models. Results showed that HQD significantly decreased fasting blood glucose, 24-h urinary protein, blood urea nitrogen, serum creatinine, and triglyceride levels in DN mice.
Front Pharmacol. 2022 Jun 21;13:934720
Sprague-Dawley rats
Normal rat model
Oral
10 mg/mL
Single dose, duration of 720 minutes
To evaluate the effect of APS on the pharmacokinetics of CAG, results showed that APS significantly increased the maximum concentration (Cmax) and area under the curve (AUC) of CAG.
Int J Nanomedicine. 2023 Nov 14;18:6705-6724
Zebrafish
Zebrafish embryos
Aqueous exposure
200–1200 µg/mL
Continuous exposure from 24 hpf to 84 hpf
To evaluate the effect of YQD on melanogenesis in zebrafish. Results showed that YQD significantly promoted melanin production in zebrafish, especially at concentrations of 400–1200 μg/mL, where melanin density was significantly higher than in the control group.
Pharmaceuticals (Basel). 2023 Dec 11;16(12):1716.
A/J mice
Lung cancer model
Oral
5.2 g/kg/d and 2.6 g/kg/d
Once daily for 28 weeks
To evaluate the therapeutic effect of AR on the lung cancer model, results showed that AR significantly reduced the number of lung tumor nodules, improved lung tissue structural integrity, and inhibited autophagy by upregulating p53 expression and downregulating p-Bcl-2 expression.
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