Biotinylated Human Siglec-15 (C-Fc-Avi, HEK293)是人源蛋白，在HEK293人源细胞中表达，带有C-Fc-Avi标签。该蛋白是Siglec家族的一种跨膜糖蛋白，包含含唾液酸结合活性的V型免疫球蛋白结构域，参与调控破骨细胞形成及TRANCE/RANK配体信号通路，在骨代谢中发挥重要作用。
Biotinylated Human Siglec-15 (C-Fc-Avi, HEK293) 化学结构 CAS号：N/A
Biotinylated Human Siglec-15 (C-Fc-Avi, HEK293) 3D分子结构 CAS号：N/A
Biotinylated Human Siglec-15 (C-Fc-Avi, HEK293) 质控信息
Biotinylated Human Siglec-15 (C-Fc-Avi, HEK293) 生物活性
描述
Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO−-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.
Lyophilized from a 0.2 μm filtered solution of PBS, 150mM NaCl, 0.3% Chaps, 5% Trehalose, pH 7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Biotinylated Human Siglec-15 (C-Fc-Avi, HEK293) 技术信息
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