货号:A2926273
同义名:
重组生物素化人LAP蛋白(N-6His-Avi, HEK293)
/ Biotinylated Human LAP (TGF-beta 1) (N-6His-Avi, HEK293); CED
Biotinylated Human LAP (TGF-beta 1) (N-6His-Avi, HEK293) 是人源蛋白,在HEK293细胞中表达,带有N端6His和Avi标签。该蛋白是TGFβ1的潜伏相关肽(LAP),与TGFβ1形成无活性复合物,其胞外激活是调控TGFβ功能的关键步骤,可作为整合素αvβ6的配体,介导局部TGFβ1活化,并与具有TGFβ调节功能的CD4+/CD25+调节性T细胞亚群相关。


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| 描述 | Transforming growth factor beta (TGFβ) family members are secreted in inactive complexes with a latency-associated peptide (LAP), a protein derived from the N-terminal region of the TGFβ gene product. Extracellular activation of these complexes is a critical step in regulation of TGFβ function in vivo. The TGFβ1 LAP is a ligand for the integrin αvβ6 and that αvβ6-expressing cells induce spatially restricted activition of TGFβ1. And LAP identifies a novel CD4+/CD25+ regulatory T cell subset with TGFbeta-mediated function and enhanced suppression of experimental autoimmune encephalomyelitis. |
| 蛋白种属 | Human |
| 表达系统 | Human cells |
| 标签 | N-6His-Avi |
| 蛋白表达区间 | Leu30-Arg278 (Cys33Ser) |
| 蛋白编号 | P01137 |
| 内毒素 | < 1 EU/µg as determined by LAL test. |
| 剂型 | 干粉 |
| 剂型描述 | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存,建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释,如果是做无血清培养或者动物体内实验时,细胞因子中不能含有BSA、FBS 和 HAS,可以使用海藻糖作为载体,然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子,并避免反复冻融;稀释分装浓度不低于 10μg/mL,分装体积不低于10μL。 分装后的蛋白在≤-20°C 下可稳定保存 3 个月。 |
| CAS号 | N/A |
| 分子量 | 31.4 KDa |
| SMILES Code | NONE |
| 别名 | 重组生物素化人LAP蛋白(N-6His-Avi, HEK293) ;Biotinylated Human LAP (TGF-beta 1) (N-6His-Avi, HEK293); CED; DPD1; LAP (TGFbeta 1); LAP (TGF-beta 1); LAP; TGFB; TGFB1; TGFbeta; transforming growth factor beta 1 |
| 运输 | 蓝冰 |
| 存储条件 |
In solvent -20°C: 3-6个月 -80°C: 12个月 Pure form Sealed in dry, store in freezer, under -20°C |
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