Biotinylated Human Frizzled-8 (C-6His-Avi, HEK293) 质控信息
Biotinylated Human Frizzled-8 (C-6His-Avi, HEK293) 生物活性
描述
Frizzled-8 is one of at least ten seven-transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co-receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. These ligands bind the extracellular CRD of Frizzled-8, blocking Wnt binding. The recombinant Frizzled-8 CRD has also been used to block Wnt signaling and inhibit growth of teratocarcinomas.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Biotinylated Human Frizzled-8 (C-6His-Avi, HEK293) 技术信息
CAS号
N/A
分子量
19.1 KDa
SMILES Code
NONE
别名
重组生物素化人Frizzled-8蛋白(C-6His-Avi, HEK293)
;Biotinylated Human Frizzled-8 (C-6His-Avi, HEK293); frizzled 8; frizzled family receptor 8; Frizzled8; Frizzled-8; FZ-8; FZD8; hFZ8
运输
蓝冰
存储条件
In solvent-20°C:3-6个月-80°C:12个月
Pure formSealed in dry, store in freezer, under -20°C
最近浏览的产品
Tags:
Biotinylated Human Frizzled-8 (C-6His-Avi, HEK293)
|Biotinylated Human Frizzled-8 (C-6His-Avi, HEK293)
| 重组生物素化人Frizzled-8蛋白(C-6His-Avi, HEK293)
| frizzled 8
| frizzled family receptor 8
| Frizzled8
| Frizzled-8
| FZ-8
| FZD8
| hFZ8
| N/A
| G-Protein-Coupled Receptors (GPCRs)
| Biotinylated Human Frizzled-8 (C-6His-Avi, HEK293) 纯度/质量文件
| Biotinylated Human Frizzled-8 (C-6His-Avi, HEK293) 生物活性
| Biotinylated Human Frizzled-8 (C-6His-Avi, HEK293) 技术信息
搜索
