/Biotinylated Human EGFR vIII (C-6His-Avi, HEK293)
Biotinylated Human EGFR vIII (C-6His-Avi, HEK293)
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货号：A2925467同义名：
重组生物素化人EGFR vIII蛋白(C-6His-Avi, HEK293)
/ Biotinylated Human EGFR vIII (C-6His-Avi, HEK293); Epidermal growth factor receptor
Biotinylated Human EGFR vIII (C-6His-Avi, HEK293) 是人源 EGFR vIII 蛋白，在人源细胞中表达，带有 C 端 6×His 和 Avi 标签。该蛋白属于 EGFR 受体酪氨酸激酶亚家族，参与配体结合后的二聚化与信号激活，调控细胞增殖、分化、迁移及凋亡等关键功能，且在多种肿瘤中高表达，是重要的抗癌药物靶点。
Biotinylated Human EGFR vIII (C-6His-Avi, HEK293) 化学结构 CAS号：N/A
Biotinylated Human EGFR vIII (C-6His-Avi, HEK293) 3D分子结构 CAS号：N/A
Biotinylated Human EGFR vIII (C-6His-Avi, HEK293) 质控信息
Biotinylated Human EGFR vIII (C-6His-Avi, HEK293) 生物活性
描述
The EGFR subfamily of receptor tyrosine kinases is composed of EGFR, ErbB2, ErbB3 and ErbB4. The EGFR shares 43% - 44% aa sequence identity with the ECD of human EGFR subfamily. All these family members are type I transmembrane glycoproteins with an extracellular ligand binding domain. The extracellular ligand binding domain is containing two cysteine-rich domains separated by a spacer region and a cytoplasmic domain containing a membrane-proximal tyrosine kinase domain. Ligand binding could induce EGFR homodimerization and heterodimerization with ErbB2, resulting in cell signaling, heterodimerization tyrosine phosphorylation and kinase activation. It can bind EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, epigen, and so on. Its signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. EGFR can also be recruited to form heterodimers with the ligand-activated ErbB3 or ErbB4. EGFR is overexpressed in different tumors. Several anti-cancer drugs use EGFR as target.
Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
复溶
Always centrifuge tubes before opening.Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.如要长期保存，建议使用含载体蛋白(如 0.1%BSA、10%FBS、5%HSA)的溶液进一步稀释，如果是做无血清培养或者动物体内实验时，细胞因子中不能含有BSA、FBS 和 HAS，可以使用海藻糖作为载体，然后分装冻存于-20℃至-80℃。注:分装保存时建议使用低吸附的管子，并避免反复冻融；稀释分装浓度不低于 10μg/mL，分装体积不低于10μL。
分装后的蛋白在≤-20°C 下可稳定保存 3 个月。
Biotinylated Human EGFR vIII (C-6His-Avi, HEK293) 技术信息
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