Berbamine, a natural product isolated and purified from the roots of Cocculus orbiculatus (L.) DC., has high binding affinity toward the (GGA)8 G-quadruplex, and is a promising agent to suppress liver cancer growth by targeting CAMKII.
Berbamine/小檗胺 细胞实验
Cell Line
Concentration
Treated Time
Description
References
PK-15 cells
0.3-5 µM
24 hours
Inhibited ASFV B646L gene transcription
J Virol. 2024 Aug 20;98(8):e0032724.
3D4/21 cells
0.4-6.4 µM
24 hours
Inhibited ASFV B646L gene transcription
J Virol. 2024 Aug 20;98(8):e0032724.
Human intestinal epithelial monolayers
36.7 μM
5 days
To evaluate the inhibitory effect of Berbamine on SARS-CoV-2 pseudovirus entry into human intestinal epithelial monolayers. Results showed that Berbamine significantly inhibited SARS-CoV-2 pseudovirus entry.
Emerg Microbes Infect. 2023 Dec;12(1):2195020.
Caco-2 cells
14.7, 7.3, 3.7 μM, or 1.8 μM
72 hours
To evaluate the inhibitory effect of Berbamine on SARS-CoV-2 pseudovirus entry into Caco-2 cells. Results showed that Berbamine significantly inhibited SARS-CoV-2 pseudovirus entry.
Emerg Microbes Infect. 2023 Dec;12(1):2195020.
Porcine alveolar macrophages (PAMs)
5 µM
72 hours
Inhibited ASFV B646L gene transcription and p72 protein expression, completely suppressed virus titer
J Virol. 2024 Aug 20;98(8):e0032724.
Vero E6 cells
0-200 μmol/L
72 hours
Assess the inhibitory effect of berbamine hydrochloride on EBOV-EGFP and wild-type MARV infection, showing effective suppression of viral replication.
Acta Pharm Sin B. 2022 Dec;12(12):4378-4389.
HEK293T cells
10 μmol/L
48 hours
Evaluate the inhibitory effect of berbamine hydrochloride on EBOV pseudovirus infection, showing significant inhibition.
Acta Pharm Sin B. 2022 Dec;12(12):4378-4389.
BHK-21 cells
15, 20 μM
24 hours
To assess the inhibitory effect of Berbamine on cell fusion
iScience. 2024 Nov 8;27(12):111347.
Caco2 cells
1.0 to 20 μM
To evaluate the antiviral activity of Berbamine against SARS-CoV-2
iScience. 2024 Nov 8;27(12):111347.
A549 cells
1.0 to 20 μM
To evaluate the antiviral activity of Berbamine against SARS-CoV-2
iScience. 2024 Nov 8;27(12):111347.
Vero-E6 cells
1.0 to 20 μM
To evaluate the antiviral activity of Berbamine against SARS-CoV-2
iScience. 2024 Nov 8;27(12):111347.
U87.LC3-mCherry-GFP cells
73.4 μM
24 hours
To evaluate the effect of Berbamine on autophagy flux. Results showed that Berbamine significantly inhibited autophagy flux.
Emerg Microbes Infect. 2023 Dec;12(1):2195020.
SMMC-7721 cells
5 μM
24 hours
BBM treatment resulted in accumulation of LC3B-II and SQSTM1, indicating autophagosome accumulation.
Cell Death Dis. 2018 Feb 14;9(2):243.
Eca109 cells
5 μM
24 hours
BBM treatment resulted in accumulation of LC3B-II and SQSTM1, indicating autophagosome accumulation.
Cell Death Dis. 2018 Feb 14;9(2):243.
A549 cells
5 μM
24 hours
BBM treatment resulted in accumulation of LC3B-II and SQSTM1, indicating autophagosome accumulation.
Cell Death Dis. 2018 Feb 14;9(2):243.
MDA-MB-231 cells
5 μM
24 hours
BBM treatment resulted in accumulation of LC3B-II and SQSTM1, indicating autophagosome accumulation.
Cell Death Dis. 2018 Feb 14;9(2):243.
MCF-7 cells
5 μM
24 hours
BBM treatment resulted in accumulation of LC3B-II and SQSTM1, indicating autophagosome accumulation.
Cell Death Dis. 2018 Feb 14;9(2):243.
Huh7 cells
10 μM
24 hours
Reduced ACE2 levels at the cell surface
Signal Transduct Target Ther. 2021 Apr 24;6(1):168.
HEK293T cells
10 μM
Inhibited the entry of SARS-CoV-2 S pseudotyped particles into cells
Signal Transduct Target Ther. 2021 Apr 24;6(1):168.
HeLa cells
10 μM
30 minutes
To evaluate the effect of Berbamine on intracellular calcium concentration. Results showed that Berbamine significantly inhibited GPN- or ML-SA1-induced lysosomal calcium release.
Emerg Microbes Infect. 2021 Dec;10(1):1257-1271.
A549 cells
1.62 μM (EC50)
48 hours
To evaluate the inhibitory effect of Berbamine on JEV infection. Results showed that Berbamine significantly inhibited JEV infection.
Emerg Microbes Infect. 2021 Dec;10(1):1257-1271.
3D4/21 cells
5 μM
24 hours
To evaluate the inhibitory effect of berbamine hydrochloride on ASFV in 3D4/21 cells, results showed that berbamine hydrochloride significantly inhibited ASFV B646L gene transcription level.
Molecules. 2022 Dec 25;28(1):170.
PK-15 cells
5 μM
24 hours
To evaluate the inhibitory effect of berbamine hydrochloride on ASFV in PK-15 cells, results showed that berbamine hydrochloride significantly inhibited ASFV B646L gene transcription level.
Molecules. 2022 Dec 25;28(1):170.
Porcine alveolar macrophages (PAMs)
0-5 μM
24 hours
To evaluate the inhibitory effect of berbamine hydrochloride on African swine fever virus (ASFV) infection, results showed that berbamine hydrochloride inhibits ASFV in a dose-dependent manner.
Molecules. 2022 Dec 25;28(1):170.
Porcine alveolar macrophages (PAMs)
50 µM
24 hours
Evaluate the antiviral activity of Berbamine against ASFV Arm/07 strain, showing significant reduction in viral titer
Virol J. 2024 Apr 25;21(1):95.
Berbamine/小檗胺 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
BALB/c mice
EBOV infection model
Oral
100 mg/kg
Once daily for 6 days
Evaluate the protective effect of berbamine hydrochloride in EBOV-infected mice, showing significant improvement in survival rate.
Acta Pharm Sin B. 2022 Dec;12(12):4378-4389.
HACE2.Tg mice
SARS-CoV-2 infection model
Oral
100 mg/kg body weight
12 h before and immediately after infection, till 6 dpi
To evaluate the prophylactic effect of Berbamine against SARS-CoV-2 infection
iScience. 2024 Nov 8;27(12):111347.
BALB/c mice
JEV infection model
Intraperitoneal injection
15 mg/kg
Twice daily for 14 days
To evaluate the protective effect of Berbamine on JEV-infected mice. Results showed that Berbamine significantly increased the survival rate (75% vs 12.5%) and alleviated brain damage.
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