Benzoylaconine is an alkaloid in the Chinese traditional medicine Radix Aconiti Lateralis Preparata (Fuzi).
Benzoylaconine/苯甲酰乌头原碱 细胞实验
Cell Line
Concentration
Treated Time
Description
References
C2C12 cells
20 μM-60 μM
1 hour
To evaluate the protective effect of BAC on H/R-induced C2C12 cell injury. Results showed that BAC dose-dependently enhanced cell viability, reduced CK and LDH levels, decreased ROS generation, and increased SOD activity.
Drug Des Devel Ther. 2024 Jun 10;18:2125-2142
HaCaT keratinocytes
0, 10, 20, 40 μM
24 h
To evaluate the effects of BAC on TNF-α/LPS-induced proliferation and inflammatory cytokine release in HaCaT cells. Results showed that BAC inhibited keratinocyte proliferation in a dose-dependent manner and reduced the expression of IL-6, IL-8, TNF-α, and IL-17.
Molecules. 2023 May 31;28(11):4473
Caco-2 cells
50 μM
6 days
To evaluate the effect of BAC on P-gp expression. Results showed that BAC at 50 μM significantly increased P-gp protein levels, with effects intermediate between AC and aconine.
Sci Rep. 2016 May 3;6:25343
HepG2 cells
25, 50, 75 μM
2 days
BAC dose-dependently increased mitochondrial mass, mtDNA copy number, cellular ATP production, and the expression of OXPHOS-related proteins.
Acta Pharmacol Sin. 2019 May;40(5):658-665
Benzoylaconine/苯甲酰乌头原碱 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Sprague-Dawley rats
Femoral artery ligation and reperfusion-induced skeletal muscle ischemia-reperfusion injury model
Intraperitoneal injection
5 mg/kg, 10 mg/kg, 20 mg/kg
Single administration before reperfusion
To evaluate the protective effect of BAC on skeletal muscle ischemia-reperfusion injury. Results showed that BAC dose-dependently alleviated muscle tissue damage, reduced tissue edema (W/D ratio), decreased serum CK and LDH levels, increased SOD activity, reduced MDA levels, and decreased apoptosis.
Drug Des Devel Ther. 2024 Jun 10;18:2125-2142
C57BL/6 mice
IMQ-induced psoriasis-like skin inflammation model
Oral administration
1 mg/kg and 3 mg/kg
Consecutive 6 days
To evaluate the therapeutic effects of BAC on IMQ-induced psoriasis-like skin inflammation in mice. Results showed that BAC significantly alleviated skin lesion symptoms, including scales, thickness, and erythema, and reduced the accumulation of Th17 cells and the release of inflammatory cytokines.
Molecules. 2023 May 31;28(11):4473
SD rats
Cardiac function evaluation model
Intravenous injection
2 mg/kg
Single dose, recorded for 30 minutes
To evaluate the effect of Benzoylaconine on cardiac function, results showed that Benzoylaconine weakened cardiac function, as represented by decreased LVSP and +dp/dt max, and increased LVEDP
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