There will be a HazMat fee per item when shipping a dangerous goods. The HazMat fee will be charged to your UPS/DHL/FedEx collect account or added to the invoice unless the package is shipped via Ground service. Ship by air in Excepted Quantity (each bottle), which is up to 1g/1mL for class 6.1 packing group I or II, and up to 25g/25ml for all other HazMat items.
Type
HazMat fee for 500 gram (Estimated)
Excepted Quantity
USD 0.00
Limited Quantity
USD 15-60
Inaccessible (Haz class 6.1), Domestic
USD 80+
Inaccessible (Haz class 6.1), International
USD 150+
Accessible (Haz class 3, 4, 5 or 8), Domestic
USD 100+
Accessible (Haz class 3, 4, 5 or 8), International
BB-Cl-amidine is a novel pan-PAD inhibitor with potency to PAD1-4, with half-life of 1.75h in vivo. BB-Cl-amidine exhibited potency against U2OS osteosarcoma cell viability with EC50 value of 8.8μM post 72-hour treatment. Inhibition of PAD by 20μM or 200μM BB-Cl-amidine could prevent PMA-induced neutrophil extracellular trap formation of bone marrow neutrophils prepared from 8-week-old MRL/lpr mice. And this effect could be observed in an in vivo study of MRL/lpr mice daily subcutaneous injected with BB-Cl-amidine at dose of 1mg/kg from 8 to 14 weeks of age. And improved vascular function, and decreasesd IFN signature in bone marrow and kidney, decreased IC deposition in kidneys and reduced proteinuria, improved skin involvement could be found after administration of BB-Cl-amidine.
BB-Cl-Amidine 细胞实验
Cell Line
Concentration
Treated Time
Description
References
U2OS cells
8.8 µM
Evaluate cytotoxicity of BB-Cl-Amidine
Acc Chem Res. 2019 Mar 19;52(3):818-832
human peripheral blood monocytes (CD14+ monocytes)
1 μM
1 h
Inhibited STING-dependent signaling, reduced IFNβ production
Proc Natl Acad Sci U S A. 2023 Aug 15;120(33):e2305420120
bone marrow-derived macrophages (BMDMs)
1 μM
1 h
Inhibited STING-dependent signaling, reduced TNF-α and IFNβ production
Proc Natl Acad Sci U S A. 2023 Aug 15;120(33):e2305420120
Primary cortical neurons
0.01, 0.05, 0.1, 0.5 nM
3 h
To evaluate the protective effect of BB-Cl-Amidine against oxygen-glucose deprivation (OGD) and NMDA-induced neuronal death. Results showed that BB-Cl-Amidine pretreatment significantly reduced OGD- and NMDA-induced neuronal death in a dose-dependent manner.
Acta Neuropathol Commun. 2025 Feb 18;13(1):33
PMNs
1-20 μM
4, 24 h
To evaluate the effect of BB-Cl-Amidine on cell viability, results showed that BB-Cl-Amidine was cytotoxic at concentrations above 1 mM
Front Immunol. 2021 Oct 19;12:716250
PBMCs
1-20 μM
24, 48, 72 h
To evaluate the effect of BB-Cl-Amidine on cell viability, results showed that BB-Cl-Amidine was cytotoxic at concentrations above 1 mM
Front Immunol. 2021 Oct 19;12:716250
CID-9 mouse mammary epithelial cells
2 µM
12 h
Inhibited PAD activity, significantly blocking prolactin-induced increase in β-casein and butyrophilin mRNA expression.
Int J Mol Sci. 2020 Apr 10;21(7):2634
monocytes
20 µM
24 h
To evaluate the effect of BB-Cl-Amidine on cell viability, results showed that BB-Cl-Amidine was cytotoxic at concentrations above 1 mM
Front Immunol. 2021 Oct 19;12:716250
NK cells
20 µM
24 h
To evaluate the effect of BB-Cl-Amidine on cell viability, results showed that BB-Cl-Amidine was cytotoxic at concentrations above 1 mM
Front Immunol. 2021 Oct 19;12:716250
B cells
20 µM
24 h
To evaluate the effect of BB-Cl-Amidine on cell viability, results showed that BB-Cl-Amidine was cytotoxic at concentrations above 1 mM
Front Immunol. 2021 Oct 19;12:716250
CD8+T cells
20 µM
24 h
To evaluate the effect of BB-Cl-Amidine on cell viability, results showed that BB-Cl-Amidine was cytotoxic at concentrations above 1 mM
Front Immunol. 2021 Oct 19;12:716250
CD4+T cells
20 µM
24 h
To evaluate the effect of BB-Cl-Amidine on cell viability, results showed that BB-Cl-Amidine was cytotoxic at concentrations above 1 mM
Front Immunol. 2021 Oct 19;12:716250
BB-Cl-Amidine 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
Trex1D18N/D18N mutant mice
Intraperitoneal injection
10 mg/kg
Daily for 8 weeks
Alleviated STING-dependent inflammatory pathology, improved survival, reduced splenomegaly and myocarditis
Proc Natl Acad Sci U S A. 2023 Aug 15;120(33):e2305420120
Sprague-Dawley rats
Middle cerebral artery occlusion (MCAO) model
Intranasal administration
5, 10, 50 µg/kg
Immediate administration (0 h) or delayed administration (48/72 h)
To evaluate the protective effect of BB-Cl-Amidine on ischemic brain injury. Results showed that BB-Cl-Amidine significantly reduced infarct volume, improved neurological and motor functions, and suppressed delayed NETosis induction.
Acta Neuropathol Commun. 2025 Feb 18;13(1):33
MRL/lpr mice
Systemic lupus erythematosus model
Subcutaneous injection
1 mg/kg/day
Once daily for 6 weeks
To evaluate the effect of BB-Cl-Amidine on NET formation and SLE-related damage, results showed that BB-Cl-Amidine significantly reduced NET formation, improved vascular function, and reduced kidney and skin damage
Ann Rheum Dis. 2015 Dec;74(12):2199-206
NOD mice
NOD mouse diabetes model
Subcutaneous injection
1 mg/g
Six times per week until 25 weeks of age
BB-Cl-amidine treatment fully prevented diabetes development in NOD mice, associated with reduced levels of citrullination in the pancreas, decreased circulating autoantibody titers against citrullinated GRP78, and reduced spontaneous neutrophil extracellular trap formation of bone marrow–derived neutrophils. Additionally, BB-Cl-amidine treatment induced a shift from Th1 to Th2 cytokines in the serum and an increase in the frequency of regulatory T cells in the blood and spleen. In the pancreas, BB-Cl-amidine treatment preserved insulin production and was associated with a less destructive immune infiltrate characterized by reduced frequencies of effector memory CD4+T cells and a modest reduction in the frequency of interferon-g–producing CD4+ and CD8+T cells.
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