7-Nitroindazole is a selective inhibitor of neuronal nitric oxide synthase (nNOS) and exhibits antinociceptive and cardiovascular effects. It serves as an important tool for studying the biological roles of nitric oxide in the central nervous system[1][2].
7-Nitroindazole/7-硝基吲唑 细胞实验
Cell Line
Concentration
Treated Time
Description
References
rat ventral hippocampus cells
1 mM
7-NI significantly increased extracellular levels of 5-HT and DA
Br J Pharmacol. 2000 Jun;130(3):575-80
Rat cremaster muscle first-order arteriole
100 μM
20 min
To investigate the constrictor effect of 7-nitroindazole on pressurized arterioles, results showed that 7-nitroindazole did not cause constriction of the arterioles.
Br J Pharmacol. 2007 Jul;151(5):602-9
7-Nitroindazole/7-硝基吲唑 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Sprague-Dawley rats
Freely moving animal model
Intraperitoneal injection
50 mg/kg
Single administration
7-NI significantly increased extracellular levels of 5-HT and DA
Br J Pharmacol. 2000 Jun;130(3):575-80
ICR mice
SP-induced itch-associated response model
Intradermal injection
10 nmol/site
Single injection
To investigate the effect of 7-Nitroindazole on SP-induced itch-associated responses. Results showed that 7-Nitroindazole significantly inhibited SP-induced scratching.
Br J Pharmacol. 2003 Jan;138(1):202-8
Mice
ENOS-deficient mice
Superfusion
100 μM
60 minutes
To investigate the role of nNOS in regulating leukocyte-endothelial cell interactions in eNOS-deficient mice. Results showed that 7-nitroindazole significantly increased leukocyte adhesion in eNOS?/? mice but had no effect in wild-type animals.
Br J Pharmacol. 2001 Sep;134(2):305-12
Male Wistar rats
6-hydroxydopamine (6-OHDA)-induced hemi-Parkinsonian model
Intraperitoneal injection
30 mg/kg
Once daily for 21 days
7-Nitroindazole pre-treatment reduced L-DOPA-induced dyskinesia and increased COX2 immunoreactivity in the dorsal striatum
Philos Trans R Soc Lond B Biol Sci. 2015 Jul 5;370(1672):20140190
Mice
Wild-type mice and NOS-1 mutant mice
Intraperitoneal injection
50 mg/kg
Single dose, experiments started 30 minutes after administration
To investigate the role of NO in short-term potentiation (STP) and long-term facilitation (LTF) of breathing. Results showed that 7-NI significantly attenuated or abolished STP and LTF.
J Physiol. 2002 Feb 15;539(Pt 1):309-15
Mice
Tail suspension model
Intraperitoneal injection
50 mg/kg
Daily for two weeks
7NI significantly prevented suspension-induced muscle atrophy, increased phosphorylated Foxo3a, and inhibited the increase in dephosphorylated and nuclear Foxo3a. Furthermore, upregulation of MuRF-1 and atrogin-1/MAFbx mRNA levels was abolished by 7NI treatment.
搜索
