Gentisic acid (GA), a natural compound, widely exists in many plants and have many biological activities, such as antioxidant, anti-inflammatory and antibiotic activities [1] . In vitro studies, it was found that gentisic acid had no cytotoxicity, but had antimutagenic activity at special concentration on HTC cells [1] . Besides this, gentisic acid could also protect rat liver mitochondria (RLM) and human erythrocytes against the oxidation and radiation [2] . In vivo studies, it was observed that gentisic acid had the ability of improving the cardiac hypertrophy and fibrosis in mice through inhibiting ERK1/2 pathway, when given 100 mg/kg/d for 3 weeks [3] .
2,5-Dihydroxybenzoic Acid 细胞实验
Cell Line
Concentration
Treated Time
Description
References
HepG2 cells
0.001, 0.01, 0.1, 1, 10 μM
24 h
To evaluate the effect of 2,5-DHBA on oleic acid-induced lipid accumulation in HepG2 cells. Results showed that 2,5-DHBA significantly reduced lipid accumulation in a dose-dependent manner without affecting cell viability.
Nutrients. 2025 May 28;17(11):1835
2,5-Dihydroxybenzoic Acid 动物实验
Species
Animal Model
Administration
Dosage
Frequency
Description
References
Mice
Bdh2 null mice
Intraperitoneal injection
500 mg/kg
Two doses, 24 hours apart, sacrificed 24 hours after last injection
Supplementation with 2,5-DHBA alleviates splenic iron overload in bdh2 null mice
Mol Cell Biol. 2014 Jul;34(13):2533-46
FVB mice
High-fat diet-induced MASLD mouse model
Oral gavage
1, 10, 100 mg/kg
Once daily for 7 days (short-term experiment) or 4 weeks (long-term experiment)
To evaluate the effect of 2,5-DHBA on hepatic lipid accumulation in HFD-induced MASLD mice. Results showed that 2,5-DHBA significantly reduced hepatic lipid accumulation in a dose-dependent manner and improved liver histology.
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