Rhodamine B Hydrazide

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Chemical Structure| 74317-53-6 同义名 : 罗丹明B
CAS号 : 74317-53-6
货号 : A615462
分子式 : C28H32N4O2
纯度 : 97%
分子量 : 456.58
MDL号 : MFCD07808762
存储条件：

Pure form Keep in dark place, inert atmosphere, 2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 : -
动物实验配方：
生物活性
描述 Rhodamine B hydrazide serves as an effective sulfite sensor, initially colorless and non-fluorescent. Its emission correlates with sulfite concentration (5-800 ng/mL; detection threshold at 1.4 ng/mL (3σ)). Sulfite converts dissolved oxygen into superoxide radicals, which react with Rhodamine B hydrazide, producing Rhodamine B and mimicking its fluorescence upon sulfite interaction, further amplified by Tween 80 surfactant micelles. Rhodamine B hydrazide exhibits peak absorption at 554 nm and fluorescence emission at 574 nm[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.19mL

0.44mL

0.22mL

10.95mL

2.19mL

1.10mL

21.90mL

4.38mL

2.19mL
参考文献

[1]Yang X F, et al. Novel spectrofluorimetric method for the determination of sulfite with rhodamine B hydrazide in a micellar medium[J]. Analytica Chimica Acta, 2002, 456(1): 121-128.