Nitidine chloride

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Chemical Structure| 13063-04-2 同义名 : Nitidine (chloride); NSC 146397
CAS号 : 13063-04-2
货号 : A524500
分子式 : C21H18ClNO4
纯度 : 97%
分子量 : 383.82
MDL号 : MFCD01659688
存储条件：

Pure form Inert atmosphere, store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 2 mg/mL(5.21 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Nitidine chloride (NC), a quaternary ammonium alkaloid, exhibits multiple biological activities, including antimalarial, antifungal, and antiangiogenesis. NC has been identified to suppress cell proliferation, stimulate apoptosis, and induce cell cycle arrest, retard migration, invasion and metastasis[3]. NC caused time- and concentration-dependent inhibition of CYP2D6, and more than 50% of CYP2D6 activity was suppressed after a 15-minute incubation with NC at 100 μM in the primary incubation mixtures, with KI of 4.36 μM, kinact of 0.052 minute-1, and a partition ratio of approximately 290[4]. NC led to the inhibition of cell growth, migration, and invasion and the induction of apoptosis, also inhibited the expression of SIN1 in osteosarcoma cells[5]. Nitidine chloride inhibited HaCaT proliferation and induced S phase cell cycle arrest. Nitidine chloride also significantly downregulated bcl-2 and upregulated bax, cleaved caspase-9 and cleaved caspase-3. In 12-O-tetradecanoyl-phorbol-13-acetate (TPA)- and imiquimod (IMQ)-induced epidermal hyperplasia and inflammation models, nitidine chloride inhibited topical edema in mouse ear and back skin, substantially reducing tissue thickness and weight[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.61mL

0.52mL

0.26mL

13.03mL

2.61mL

1.30mL

26.05mL

5.21mL

2.61mL
参考文献

[1]Fang Z, Tang Y, et al. Nitidine chloride inhibits renal cancer cell metastasis via suppressing AKT signaling pathway. Food Chem Toxicol. 2013 Oct;60:246-51.

[2]Liu M, Wang J, et al. Nitidine chloride inhibits the malignant behavior of human glioblastoma cells by targeting the PI3K/AKT/mTOR signaling pathway. Oncol Rep. 2016 Oct;36(4):2160-8.

[3]Cui Y, Wu L, Cao R, Xu H, Xia J, Wang ZP, Ma J. Antitumor functions and mechanisms of nitidine chloride in human cancers. J Cancer. 2020 Jan 1;11(5):1250-1256

[4]Mao X, Hu Z, Wang Q, Zhang N, Zhou S, Peng Y, Zheng J. Nitidine Chloride Is a Mechanism-Based Inactivator of CYP2D6. Drug Metab Dispos. 2018 Aug;46(8):1137-1145

[5]Xu H, Cao T, Zhang X, Shi Y, Zhang Q, Chai S, Yu L, Jin G, Ma J, Wang P, Li Y. Nitidine Chloride Inhibits SIN1 Expression in Osteosarcoma Cells. Mol Ther Oncolytics. 2019 Feb 5;12:224-234

[6]Yang XG, Jiang BW, Jing QQ, Li WJ, Tan LP, Bao YL, Song ZB, Yu CL, Liu L, Liu YC, Li YX. Nitidine chloride induces S phase cell cycle arrest and mitochondria-dependent apoptosis in HaCaT cells and ameliorates skin lesions in psoriasis-like mouse models. Eur J Pharmacol. 2019 Nov 15;863:172680