NSC632839

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Chemical Structure| 157654-67-6 同义名 : Ubiquitin Isopeptidase Inhibitor II; F6
CAS号 : 157654-67-6
货号 : A457204
分子式 : C21H22ClNO
纯度 : 99%+
分子量 : 339.86
MDL号 : MFCD09753281
存储条件：

Pure form Sealed in dry, 2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 3 mg/mL(8.83 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
生物活性
靶点

  • USP/UBP

    USP7, EC50:37 μM

    USP2, EC50:45 μM
描述 Deubiquitylases (DUBs), isopeptidases that cleave at the carboxyl terminus of ubiquitin, have been divided into five distinct groups, the largest being the ubiquitin-specific proteases (USP/UBP). NSC 632839 is an inhibitor of DUB with IC50 values of 45 nM, 37 nM and 9.8 nM for USP2, USP7, and SENP2, respectively[3]. In vitro, treatment with 10 μM NSC 632839 activated caspase-3/caspase-7 and triggered apoptosis in IMR90-E1A and E1A/C9DN cells. NSC 632839 inhibited IMR90-E1A and E1A/C9DN cells proliferation with IC50 values of 15.65 μM and 16.23 μM[4]. Treatment with 10 μM NSC 632839 strongly induced LC3 puncta formation in Hela cells[5].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.94mL

0.59mL

0.29mL

14.71mL

2.94mL

1.47mL

29.42mL

5.88mL

2.94mL
参考文献

[1]Aleo E, Henderson CJ, et al. Identification of new compounds that trigger apoptosome-independent caspase activation and apoptosis. Cancer Res. 2006 Sep 15;66(18):9235-44.

[2]Nicholson B, Leach CA, et al. Characterization of ubiquitin and ubiquitin-like-protein isopeptidase activities. Protein Sci. 2008 Jun;17(6):1035-43.

[3]Nicholson B, Leach CA, Goldenberg SJ, Francis DM, Kodrasov MP, Tian X, Shanks J, Sterner DE, Bernal A, Mattern MR, Wilkinson KD, Butt TR. Characterization of ubiquitin and ubiquitin-like-protein isopeptidase activities. Protein Sci. 2008 Jun;17(6):1035-43.

[4]Aleo E, Henderson CJ, Fontanini A, Solazzo B, Brancolini C. Identification of new compounds that trigger apoptosome-independent caspase activation and apoptosis. Cancer Res. 2006 Sep 15;66(18):9235-44.

[5]Yan C, Huo H, Yang C, Zhang T, Chu Y, Liu Y. Ubiquitin C-Terminal Hydrolase L1 regulates autophagy by inhibiting autophagosome formation through its deubiquitinating enzyme activity. Biochem Biophys Res Commun. 2018 Mar 4;497(2):726-733.