Hygromycin B

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Chemical Structure| 31282-04-9 同义名 : 潮霉素 ;Hygrovetine
CAS号 : 31282-04-9
货号 : A446642
分子式 : C20H37N3O13
纯度 : ≥950U/mg
分子量 : 527.52
MDL号 : MFCD06795479
存储条件：

Pure form Keep in dark place, inert atmosphere, 2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(199.04 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

H2O: 50 mg/mL(94.78 mM)，配合低频超声助溶
生物活性
描述 Hygromycin B is an aminocyclitol antibiotic that strongly inhibits both 70S and 80S ribosomes, produced by streptomyces hygroscopicus. Hygromycin B inhibits protein synthesis in archaebacteria, eubacteria, fungi, and higher eukaryotes[3]. in vitro，hygromycin B at 0.38 mM concentration completely halts yeast cell growth in rich media, presumably by preventing protein synthesis by cytoplasmic ribosomes[4]. in vivo，hygromycin B inhibits protein synthesis by blocking ribosomal translocation without causing significant misreading[3]. In eukaryotes, the antibiotic affects EF-2-mediated translocation of A site bound tRNA to the P site. The inhibition of translocation is accompanied by an increase in the affinity of the A site for aminoacyl-tRNA[5].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.90mL

0.38mL

0.19mL

9.48mL

1.90mL

0.95mL

18.96mL

3.79mL

1.90mL
参考文献

[1]Brodersen DE, Clemons WM Jr, et al. The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit. Cell. 2000 Dec 22;103(7):1143-54.

[2]Pardo JM, Malpartida F, et al. Biochemical basis of resistance to hygromycin B in Streptomyces hygroscopicus--the producing organism. J Gen Microbiol. 1985 Jun;131(6):1289-98.

[3]Pfister P, Risch M, Brodersen DE, Böttger EC. Role of 16S rRNA Helix 44 in Ribosomal Resistance to Hygromycin B. Antimicrob Agents Chemother. 2003;47(5):1496-1502.

[4]González A, Jiménez A, Vázquez D, Davies JE, Schindler D. Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. Biochim Biophys Acta. 1978;521(2):459-469.

[5]Brodersen DE, Clemons WM Jr, Carter AP, Morgan-Warren RJ, Wimberly BT, Ramakrishnan V. The structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin B on the 30S ribosomal subunit. Cell. 2000;103(7):1143-1154.

[6]Macintyre, G et al. “Hygromycin B therapy of a murine coronaviral hepatitis.” Antimicrobial agents and chemotherapy vol. 35,10 (1991): 2125-7.