Dexamethasone

产品说明书

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Chemical Structure| 50-02-2 同义名 : Prednisolone F; Hexadecadrol; NSC 34521; MK-125
CAS号 : 50-02-2
货号 : A144976
分子式 : C22H29FO5
纯度 : 97%
分子量 : 392.46
MDL号 : MFCD00064136
存储条件：

Pure form Sealed in dry, 2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 250 mg/mL(637.01 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO

无水乙醇: 8 mg/mL(20.38 mM)，配合低频超声助溶，注意：无水乙醇开封后，易挥发，也会吸收空气中的水分，导致溶解能力下降，请避免使用开封较久的乙醇
动物实验配方：

5% DMSO+45% PEG 300+water 13 mg/mL

生物活性
靶点

  • IL Receptor
描述 Dexamethasone是一种强效合成糖皮质激素，通过高亲和力结合糖皮质激素受体（GR），诱导SGK1介导的GDI磷酸化，增强Rab蛋白依赖的AMPAR膜转运，调控海马与杏仁核突触可塑性。显著抑制NF-κB通路，阻断促炎因子（TNF-α, IL-6）释放，并抑制树突状细胞分化，发挥超强免疫抑制与抗炎作用（效力为氢化可的松的25倍）。
*该产品仅可用于细胞层面的研究，不可用于动物实验。
细胞研究
细胞系 浓度 检测类型 检测时间 活性说明 数据源
293 MSR cells Function assay 60 mins Displacement of [3H]-dexamethasone human glucocorticoid receptor alpha expressed in 293 MSR cells after 60 mins by scintillation counting, EC50=12.5 nM 23199485
african green monkey COS7 cells Function assay Agonist activity at glucocorticoid receptor ligand binding domain expressed in african green monkey COS7 cells co-transfected with Gal4-LBD by luciferase reporter gene assay, EC50=7.2 nM 19863083
COS7 cells 1 μM Function assay Agonist activity at glucocorticoid receptor Q570A mutant in COS7 cells at 1 uM by NF-kappaB-luciferase reporter gene assay 18038970
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

2.55mL

0.51mL

0.25mL

12.74mL

2.55mL

1.27mL

25.48mL

5.10mL

2.55mL
参考文献

[1]Poulsen RC, Watts AC, Murphy RJ, Snelling SJ, Carr AJ, Hulley PA. Glucocorticoids induce senescence in primary human tenocytes by inhibition of sirtuin 1 and activation of the p53/p21 pathway: in vivo and in vitro evidence. Ann Rheum Dis. 2014 Jul;73(7):1405-13.

[2]Fouty B, Moss T, Solodushko V, Kraft M. Dexamethasone can stimulate G1-S phase transition in human airway fibroblasts in asthma. Eur Respir J. 2006 Jun;27(6):1160-7.

[3]Muhammad W, Zhu J, Zhai Z, Xie J, Zhou J, Feng X, Feng B, Pan Q, Li S, Venkatesan R, Li P, Cao H, Gao C. ROS-responsive polymer nanoparticles with enhanced loading of dexamethasone effectively modulate the lung injury microenvironment. Acta Biomater. 2022 Aug;148:258-270.