Osthole

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Chemical Structure| 484-12-8 同义名 : Osthol; NSC 31868; Ostol; Ostole; 7-Methoxy-8-(3-methyl-2-butenyl)coumarin
CAS号 : 484-12-8
货号 : A141553
分子式 : C15H16O3
纯度 : 98%
分子量 : 244.29
MDL号 : MFCD00076049
存储条件：

Pure form Sealed in dry,2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 105 mg/mL(429.82 mM)，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
动物实验配方：
生物活性
描述 Osthole, a natural coumarin found in traditional Chinese medicinal herbs, has therapeutic potential in the treatment of various diseases. Mice were administered osthole (100 mg/kg/d, ip) for 3 d, then on the fourth day APAP (Acetaminophen) (300 mg/kg, ip) was co-administered with osthole. Compared with the mice treated with APAP alone, osthole pretreatment significantly reduced serum MDA levels and hepatic H2O2 levels, and improved liver GSH levels and the GSSG-to-GSH ratio. In cultured murine primary hepatocytes and Raw264.7 cells, however, osthole (40 μM) did not alleviate APAP-induced cell death, but it significantly suppressed APAP-caused elevation of inflammatory cytokines[3]. Mice were injected with osthole (100 mg/kg, ip) or vehicle, followed by TMX (Tamoxifen, 90 mg/kg, ip) 24 h later. Osthole prevents TMX hepatotoxicity by suppressing p38 activation and subsequently reducing TMX-induced oxidative damage[4]. Pretreatment with osthole (100 mg/kg/d, ip, 3d) significantly ameliorated the clinical scores, colon length shortening, colonic histopathological changes and the expression of inflammatory mediators in TNBS-induced colitis. In mouse peritoneal macrophages, pretreatment with osthole (50 μM) significantly attenuated the LPS-induced elevation of cytokines at the mRNA level[5]. Osthole can be considered as a potential component of the treatment of Osteoarthritis, for it possesses a cartilage protective effect, as well as anti-inflammation, analgesic, and movement improving effects[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

4.09mL

0.82mL

0.41mL

20.47mL

4.09mL

2.05mL

40.93mL

8.19mL

4.09mL
参考文献

[1]Kuo PL, Hsu YL, et al. Osthole-mediated cell differentiation through bone morphogenetic protein-2/p38 and extracellular signal-regulated kinase 1/2 pathway in human osteoblast cells. J Pharmacol Exp Ther. 2005 Sep;314(3):1290-9.

[2]Ko FN, Wu TS, et al. Inhibition of platelet thromboxane formation and phosphoinositides breakdown by osthole from Angelica pubescens. Thromb Haemost. 1989 Nov 24;62(3):996-9.

[3]Cai Y, Sun W, Zhang XX, Lin YD, Chen H, Li H. Osthole prevents acetaminophen-induced liver injury in mice. Acta Pharmacol Sin. 2018 Jan;39(1):74-84

[4]Zhou WB, Zhang XX, Cai Y, Sun W, Li H. Osthole prevents tamoxifen-induced liver injury in mice. Acta Pharmacol Sin. 2019 May;40(5):608-619

[5]Sun W, Cai Y, Zhang XX, Chen H, Lin YD, Li H. Osthole pretreatment alleviates TNBS-induced colitis in mice via both cAMP/PKA-dependent and independent pathways. Acta Pharmacol Sin. 2017 Aug;38(8):1120-1128

[6]Chern CM, Zhou H, Wang YH, Chang CL, Chiou WF, Chang WT, Yao CH, Liou KT, Shen YC. Osthole ameliorates cartilage degradation by downregulation of NF-κB and HIF-2α pathways in an osteoarthritis murine model. Eur J Pharmacol. 2020 Jan 15;867:172799