cGAMP

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Chemical Structure| 849214-04-6 同义名 : Cyclic GMP-AMP; 3',3'-cGAMP
CAS号 : 849214-04-6
货号 : A982224
分子式 : C20H24N10O13P2
纯度 : 98%+
分子量 : 674.41
MDL号 : N/A
存储条件：

Pure form Keep in dark place, inert atmosphere, store in freezer, under -20°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

H2O: 150 mg/mL(222.42 mM)，配合低频超声助溶
生物活性
描述 cGAMP (cyclic-GMP-AMP) functions as an endogenous second messenger that triggers interferon production in response to cytosolic DNA. It is a STING ligand which binds to STING, leading to the activation of IRF3 and induction of interferon-β[4]. cGAS-synthesized cGAMP is transferred from producing cells to neighbouring cells through gap junctions, where it promotes STING activation and thus antiviral immunity independently of type I IFN signaling, identifying cGAS-triggered cGAMP transfer as a novel host strategy that serves to rapidly convey antiviral immunity in a transcription-independent, horizontal manner[5]. On stimulation with cGAMP, fibroblasts from the patients showed increased transcription of IFNB1[6].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.48mL

0.30mL

0.15mL

7.41mL

1.48mL

0.74mL

14.83mL

2.97mL

1.48mL
参考文献

[1]Wu J, Sun L, et al. Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA. Science. 2013 Feb 15;339(6121):826-30.

[2]Ablasser A, Schmid-Burgk JL, et al. Cell intrinsic immunity spreads to bystander cells via the intercellular transfer of cGAMP. Nature. 2013 Nov 28;503(7477):530-4.

[3]Liu Y, Jesus AA, et al. Activated STING in a vascular and pulmonary syndrome. N Engl J Med. 2014 Aug 7;371(6):507-518.

[4]Wu J, Sun L, Chen X, Du F, Shi H, Chen C, Chen ZJ. Cyclic GMP-AMP is an endogenous second messenger in innate immune signaling by cytosolic DNA. Science. 2013 Feb 15;339(6121):826-30. doi: 10.1126/science.1229963. Epub 2012 Dec 20. PMID: 23258412; PMCID: PMC3855410.

[5]Ablasser A, Schmid-Burgk JL, Hemmerling I, Horvath GL, Schmidt T, Latz E, Hornung V. Cell intrinsic immunity spreads to bystander cells via the intercellular transfer of cGAMP. Nature. 2013 Nov 28;503(7477):530-4. doi: 10.1038/nature12640. Epub 2013 Sep 29. PMID: 24077100; PMCID: PMC4142317.

[6]Liu Y, Jesus AA, Marrero B, Yang D, Ramsey SE, Sanchez GAM, Tenbrock K, Wittkowski H, Jones OY, Kuehn HS, Lee CR, DiMattia MA, Cowen EW, Gonzalez B, Palmer I, DiGiovanna JJ, Biancotto A, Kim H, Tsai WL, Trier AM, Huang Y, Stone DL, Hill S, Kim HJ, St Hilaire C, Gurprasad S, Plass N, Chapelle D, Horkayne-Szakaly I, Foell D, Barysenka A, Candotti F, Holland SM, Hughes JD, Mehmet H, Issekutz AC, Raffeld M, McElwee J, Fontana JR, Minniti CP, Moir S, Kastner DL, Gadina M, Steven AC, Wingfield PT, Brooks SR, Rosenzweig SD, Fleisher TA, Deng Z, Boehm M, Paller AS, Goldbach-Mansky R. Activated STING in a vascular and pulmonary syndrome. N Engl J Med. 2014 Aug 7;371(6):507-518. doi: 10.1056/NEJMoa1312625. Epub 2014 Jul 16. PMID: 25029335; PMCID: PMC4174543.