Cresyl Violet acetate
产品说明书
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同义名 : | 甲酚紫醋酸盐 |
| CAS号 : | 10510-54-0 | |
| 货号 : | A900422 | |
| 分子式 : | C18H15N3O3 | |
| 纯度 : | 65% | |
| 分子量 : | 321.33 | |
| MDL号 : | MFCD00013151 | |
| 存储条件： |
Pure form Keep in dark place,Sealed in dry,2-8°C In solvent -20°C:3-6个月-80°C:12个月 |
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| 溶解度 : |
DMSO: 1 mg/mL(3.11 mM)，配合低频超声助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO |
| 生物活性 | |||
|---|---|---|---|
| 描述 | Cresyl violet acetate, also known as oxazine-9, is commonly used for Nissl staining of spinal cord and brain sections, and is used to visualize cell nuclei and Nissl bodies in neuroanatomical studies. Simple protocol De-wax sections in xylene (2 or 3 changes of 3min each) Rehydrate in alcohol (100% x2), 3min each. Stain in 0.1% Cresyl Violet 4-15min. Quick rinse in tap water to remove excess stain Wash in 70% ethanol (the stain will be removed by this method). If required immerse sections for 2min in Differentiation solution – check staining on microscope. Dehydrate through 2x3min changes of absolute ethanol. Clear in xylene x2 and mount in DePeX. Allow dry in the fume hood[1]. | ||
| 实验方案 | |||
|---|---|---|---|
| 1mg | 5mg | 10mg | |
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1 mM 5 mM 10 mM |
3.11mL 0.62mL 0.31mL |
15.56mL 3.11mL 1.56mL |
31.12mL 6.22mL 3.11mL |
| 参考文献 |
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