JC-1

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Chemical Structure| 3520-43-2 同义名 : CBIC2; NK 1420
CAS号 : 3520-43-2
货号 : A659203
分子式 : C25H27Cl4IN4
纯度 : 99%+
分子量 : 652.23
MDL号 : MFCD00188475
存储条件：

Pure form Keep in dark place,Sealed in dry,2-8°C

In solvent -20°C:3-6个月-80°C:12个月
溶解度 :

DMSO: 4 mg/mL(6.13 mM)，配合低频超声，并水浴加热至45℃助溶，注意：DMSO长时间开封后，会吸水并导致溶解能力下降，请避免使用长期开封的DMSO
生物活性
描述 JC-1 (CBIC2) is an ideal fluorescent probe for detecting mitochondrial membrane potential, accumulating in mitochondria in a potential-dependent manner. It can be used to assess the membrane potential of cells, tissues, or purified mitochondria. In healthy mitochondria, JC-1 aggregates in the mitochondrial matrix, forming a polymer that emits strong red fluorescence; when mitochondrial membrane potential decreases, JC-1 cannot aggregate, producing green fluorescence instead[1].
实验方案
1mg 5mg 10mg

1 mM

5 mM

10 mM

1.53mL

0.31mL

0.15mL

7.67mL

1.53mL

0.77mL

15.33mL

3.07mL

1.53mL
参考文献

[1]A Perelman, et al. JC-1: alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry. Cell Death Dis. 2012 Nov 22;3:e430.